Protein Info for PS417_26835 in Pseudomonas simiae WCS417

Annotation: 3-mercaptopyruvate sulfurtransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 287 PF00581: Rhodanese" amino acids 8 to 131 (124 residues), 55.7 bits, see alignment E=2.9e-19 amino acids 160 to 273 (114 residues), 48.9 bits, see alignment E=3.8e-17

Best Hits

Swiss-Prot: 67% identical to THTM_PSEAE: Probable 3-mercaptopyruvate sulfurtransferase (sseA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01011, thiosulfate/3-mercaptopyruvate sulfurtransferase [EC: 2.8.1.1 2.8.1.2] (inferred from 88% identity to pfs:PFLU5786)

Predicted SEED Role

"Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1)" (EC 2.8.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.1, 2.8.1.2

Use Curated BLAST to search for 2.8.1.1 or 2.8.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UGQ4 at UniProt or InterPro

Protein Sequence (287 amino acids)

>PS417_26835 3-mercaptopyruvate sulfurtransferase (Pseudomonas simiae WCS417)
MPLAQLISPQQLAERQKAASVVILDCRFALEDPDYGRCSYAEGHIEGAQYADLNRHLSGT
VIKGVTGRHPLPAAETLVQRLRAWGISADTDIVLYDDGPGAYAARAWWLLAWLGKRDGVF
ILDGGLKAWHGAGFPLSLDAPLVAPGTFAGTPDNRLLLDAEQLEKRLGQPELTLIDARAQ
PRFRGDVEPIDPIAGHIPGAQCAAFNENLDTDGRFLPAAQLKQRFAAQLQGRSPDELVAY
CGSGVTACHNLFALSLAGYPLGKLYAGSWSEWITDPKREIATGDSVS