Protein Info for GFF5229 in Variovorax sp. SCN45
Annotation: Guanine deaminase (EC 3.5.4.3); Hydroxydechloroatrazine ethylaminohydrolase (EC 3.5.99.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 49% identical to OXODE_PSEAE: 8-oxoguanine deaminase (PA0142) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K03382, hydroxyatrazine ethylaminohydrolase [EC: 3.5.99.3] (inferred from 93% identity to vpe:Varpa_1145)MetaCyc: 49% identical to 8-oxoguanine deaminase monomer (Pseudomonas aeruginosa)
RXN-11455 [EC: 3.5.4.32]
Predicted SEED Role
"Guanine deaminase (EC 3.5.4.3); Hydroxydechloroatrazine ethylaminohydrolase (EC 3.5.99.3)" (EC 3.5.4.3, EC 3.5.99.3)
MetaCyc Pathways
- purine nucleotides degradation I (plants) (10/12 steps found)
- guanosine nucleotides degradation II (4/4 steps found)
- purine nucleotides degradation II (aerobic) (9/11 steps found)
- superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
- guanosine nucleotides degradation III (3/4 steps found)
- drosopterin and aurodrosopterin biosynthesis (5/7 steps found)
- superpathway of purines degradation in plants (12/18 steps found)
- purine nucleobases degradation II (anaerobic) (16/24 steps found)
- purine nucleobases degradation I (anaerobic) (6/15 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.5.4.3
Use Curated BLAST to search for 3.5.4.3 or 3.5.4.32 or 3.5.99.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (480 amino acids)
>GFF5229 Guanine deaminase (EC 3.5.4.3); Hydroxydechloroatrazine ethylaminohydrolase (EC 3.5.99.3) (Variovorax sp. SCN45) MGPNDIMNTSTATTPSPLRIVRHADVLVTMDAARREIRDGAVVIQGPAIAWVGATAELPA AYLDALRNGNAEAIDMHGHVVMPGLVNTHHHMYQSLTRAVPEAQDAELFGWLTNLYLLWA RLTPEMIRVSTQTAMAELMLSGCTTTSDHLYLFPNGSRLDDSIEAADAMGMRFHAARGSM SVGRSAGGLPPDEVVETEEAILRDSERLIGRWHDASCHSMRRIVLAPCSPFSVSRDLMRL SAELARERGVSLHTHLAENDNDVAYSREKFGMTPAEYAEDLGWVGRDVWHAHCVKLDEAG IALFGRTGTGVAHCPCSNMRLASGIAPIGAMRRAGVPVGLGVDGSASNDGAHMLGEARQA MLLQRVGYGPAAMTAREALEIATLGGARVLGRDDIGALAPGMSADIVAFDMRGAAHAGAG HDPVAALVFCTPASVSLSVIGGTVRVRGGEFTGLELAPLLARHRDLARTLYEAARHPHTA