Protein Info for GFF5228 in Variovorax sp. SCN45

Annotation: Arachidonate 15-lipoxygenase precursor (EC 1.13.11.33)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Predicted SEED Role

"Arachidonate 15-lipoxygenase precursor (EC 1.13.11.33)" (EC 1.13.11.33)

MetaCyc Pathways

anandamide lipoxygenation (4/6 steps found)
protectin biosynthesis (3/6 steps found)
lipoxin biosynthesis (6/11 steps found)
α-linolenate metabolites biosynthesis (1/7 steps found)
resolvin D biosynthesis (1/11 steps found)
peptido-conjugates in tissue regeneration biosynthesis (5/17 steps found)
representative whole plasma arachidonate-derived icosanoids I (1/13 steps found)
di-homo-γ-linolenate metabolites biosynthesis (1/16 steps found)
representative whole plasma arachidonate-derived icosanoids II (1/19 steps found)
docosahexaenoate metabolites biosynthesis (5/31 steps found)
icosapentaenoate metabolites biosynthesis (5/35 steps found)
arachidonate metabolites biosynthesis (4/74 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.11.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (669 amino acids)

>GFF5228 Arachidonate 15-lipoxygenase precursor (EC 1.13.11.33) (Variovorax sp. SCN45)
MKRRDILKWSASAGGLSLLAAKPSTASAALPVLKPTLPQKIDPISATLRRLELVGRQATY
LWTESHINLAGVPMGAVVPATELPALEHQLKTIAKAVEAVTNFAASFATSALASGSLQSL
DNLRTRLAALQASFDQLLAANGGLLTLPAAALSLLGTLTGTAGDIATQLKQIHHDLLSQG
PLGALNGPKTVAQYDALFVTIDKPAVAQLLHDNDLFAYMRVGGPNPMLIKRATALPAKFP
LGDAQYRQVMGADDSLADAAASGRLYLLDYEGLGDMAPTGPVSKPLTGTGYVYAPVALFA
RPKTGRSLVPVAIQCGQDPALNPIFLRVDDTANAEAYWAWQSAKTAVQVADFNYHEMFVH
LGRTHLMSEAFAMATQRQFATAHPLSRLLSPHLEGAMFINEAATLIIMAPLTTGDVILTA
PIETLQQQCGRDRLAYDFYDRMMLPNDLRTRGVDDTAALPDYPYRDDALLVWNTIAQWVG
DYVAAYYLGDGDVTGDYELQAWATELATSGKVHGFRPVTTRAQLVDVLTAIIFNASAQHA
AVNFPQYSVMTYAPFSAGAGGTPAPTSNAGQSEASWSQMLPSRLGAQEQILLFHILGGVY
YRPLGEYKDNVFPHLPVLLDPAISGQGGPLERFRNALAGIENTIRQRNTARSRPYEHLLP
SRIPSSTNI