Protein Info for PS417_26760 in Pseudomonas simiae WCS417

Annotation: oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 TIGR00539: putative oxygen-independent coproporphyrinogen III oxidase" amino acids 28 to 363 (336 residues), 401.6 bits, see alignment E=1.6e-124 PF04055: Radical_SAM" amino acids 31 to 199 (169 residues), 86.1 bits, see alignment E=3.2e-28 PF06969: HemN_C" amino acids 325 to 390 (66 residues), 47.2 bits, see alignment E=1.9e-16

Best Hits

KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 97% identity to pfs:PFLU5770)

Predicted SEED Role

"Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB" in subsystem Heat shock dnaK gene cluster extended or Heme and Siroheme Biosynthesis or Queuosine-Archaeosine Biosynthesis

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.22

Use Curated BLAST to search for 1.3.99.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1STE6 at UniProt or InterPro

Protein Sequence (400 amino acids)

>PS417_26760 oxidase (Pseudomonas simiae WCS417)
MTQNTSAQPLIHGGAQTPRAALPVLPPLSLYIHIPWCVRKCPYCDFNSHTASKVLPEEEY
VDALLADLDQDLHAVYGRELSSIFFGGGTPSLFSAAALGRLLKGVQARIPFASDIEITLE
ANPGTFEQEKFVAYRTLGINRLSIGIQSFQQEKLQALGRIHNGDEAVRAAGMARQAGFDN
FNLDLMHGLPDQSLDDALSDLRQAVALRPTHLSWYQLTLEPNTVFWNQPPALPEDDTLWD
IQEAGQALLAEHGYAQYEVSAYAQPGRPARHNLNYWSFGDFIGIGAGAHGKLSHPDGRIV
RTWKTRAPKDYLNPAKSFQAGAKELTNEELPFEFLMNALRLTDGVEAKLYAERTGLELAS
LDEGRREAEQSGLMQVEPSRLAATDRGQLFLNDLLQKFLS