Protein Info for GFF5220 in Sphingobium sp. HT1-2

Annotation: Sodium-transporting ATPase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 233 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details amino acids 78 to 98 (21 residues), see Phobius details amino acids 112 to 131 (20 residues), see Phobius details amino acids 144 to 156 (13 residues), see Phobius details amino acids 165 to 191 (27 residues), see Phobius details amino acids 199 to 219 (21 residues), see Phobius details PF00119: ATP-synt_A" amino acids 25 to 214 (190 residues), 139.3 bits, see alignment E=8.6e-45

Best Hits

Swiss-Prot: 59% identical to ATP62_BRASB: ATP synthase subunit a 2 (atpB2) from Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)

KEGG orthology group: K02108, F-type H+-transporting ATPase subunit a [EC: 3.6.3.14] (inferred from 63% identity to avn:Avin_19710)

Predicted SEED Role

"ATP synthase F0 sector subunit a"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (233 amino acids)

>GFF5220 Sodium-transporting ATPase subunit B (Sphingobium sp. HT1-2)
MKFASPLESHVLFAIGPVPISQPVATTWGIMAVLTIGSWLLTRRLAFRPTQAQAALELVV
ETIAGQIRETMEAEPRRYLPLIGTLFLFLLVANWSSLIPGVEPPTATLETDAALALIVFG
ATLWFGVRALGPRAYLATFARPSILLAPLNIVELFTRSFSLMVRLFGNVMSGVFMIGVVL
SLAGLLVPIPLMALEMLTGAVQAYIFGALAMVFIGSAISENGARPPSKQESPA