Protein Info for PS417_26675 in Pseudomonas simiae WCS417

Annotation: glutathione synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 317 TIGR01380: glutathione synthase" amino acids 3 to 313 (311 residues), 448 bits, see alignment E=7.7e-139 PF02951: GSH-S_N" amino acids 4 to 121 (118 residues), 158.8 bits, see alignment E=8.7e-51 PF02955: GSH-S_ATP" amino acids 125 to 297 (173 residues), 261.9 bits, see alignment E=3.1e-82 PF08443: RimK" amino acids 151 to 310 (160 residues), 38.7 bits, see alignment E=1.2e-13

Best Hits

Swiss-Prot: 87% identical to GSHB_PSESM: Glutathione synthetase (gshB) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K01920, glutathione synthase [EC: 6.3.2.3] (inferred from 99% identity to pfs:PFLU5753)

MetaCyc: 67% identical to glutathione synthetase (Escherichia coli K-12 substr. MG1655)
Glutathione synthase. [EC: 6.3.2.3]

Predicted SEED Role

"Glutathione synthetase (EC 6.3.2.3)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Heat shock dnaK gene cluster extended (EC 6.3.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UAH1 at UniProt or InterPro

Protein Sequence (317 amino acids)

>PS417_26675 glutathione synthetase (Pseudomonas simiae WCS417)
MSVRVGIVMDPIASISYKKDSSLAMLLAAQARGWTLFYMEQRDLYQGDGEARARMRPLQV
FANPQKWFELADEIDSPLSDLDVILMRKDPPFDMEFVYSTYLLEQAERAGVLIVNKPQSL
RDCNEKLFATLFPQCTPPTVVSRRADVLREFAAKHGDVILKPLDGMGGTSIFRHRAGDPN
LSVILETLTALGTQQIMGQAYLPAIKDGDKRILMIDGEPVDYCLARIPAAGETRGNLAAG
GRGEARPLSDKDRWIAAQVGPTLREKGLLFVGLDVIGEHLTEINVTSPTCIREIDNAFGT
NIGEMLMAAIERKLQAK