Protein Info for GFF5208 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 561 PF13450: NAD_binding_8" amino acids 22 to 67 (46 residues), 25.1 bits, see alignment 5.3e-09 PF21162: ETFQO_UQ-bd" amino acids 221 to 314 (94 residues), 146.5 bits, see alignment E=9e-47 PF05187: ETF_QO" amino acids 457 to 558 (102 residues), 168.8 bits, see alignment E=8.8e-54

Best Hits

Swiss-Prot: 51% identical to ETFD_DICDI: Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial (etfdh) from Dictyostelium discoideum

KEGG orthology group: K00311, electron-transferring-flavoprotein dehydrogenase [EC: 1.5.5.1] (inferred from 81% identity to pol:Bpro_1895)

Predicted SEED Role

"Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases (EC 1.5.5.1)

Isozymes

Compare fitness of predicted isozymes for: 1.5.5.1

Use Curated BLAST to search for 1.5.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (561 amino acids)

>GFF5208 Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTPEEILSTYGPREAMEYDVVVVGGGPGGLATAIRLKQIAAEKGGDINVCVLEKGSEPGA
HILSGAVMDPKALNELLPNWKADGAPLNQPVTDDKLLWLTEKGAIDTPKFLIPNNFHNEG
NYVVGLGNVVKWLGQQAENLGVEIFPGFAAAEVLYNDDGSVKGVATGNLGVGKDGEPTDN
FQLGMELHAKYTVFAEGARGHLGKRLIAKFHLDEGKDPQAYAIGVKELWEIDPAKHQAGL
VIHTAGWPMKDDTYGGGFMYHLEDNKVTLGLVTGLNYSNPWLSPFEEMQRWKTHPRIRAF
LEGGKRISYGARAITTGGLMSLPQTTFPGGALVGCDAGYLNGARIKGSHAAIKSGMLCAE
AAFEAVQAGRQHDELKAYPAAFDKSWLREELDASRNFKSWFKYGNIVGGLMVGVEHWLLP
RLGFKAPPWSLHREKADHLYLKPAAECEQIVYPKPDGKLTFDRLGSVFVSNTNHEEHQPA
HLTLKDASVPVKINLAKYAGPEARYCPAGVYEFVKNPDNTDRLQINAQNCVHCKTCDIKD
PTQNIVWVTPEGGGGPNYAGM