Protein Info for GFF5207 in Sphingobium sp. HT1-2

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 785 transmembrane" amino acids 132 to 152 (21 residues), see Phobius details amino acids 162 to 182 (21 residues), see Phobius details amino acids 194 to 217 (24 residues), see Phobius details amino acids 232 to 251 (20 residues), see Phobius details amino acids 388 to 409 (22 residues), see Phobius details amino acids 415 to 437 (23 residues), see Phobius details amino acids 731 to 750 (20 residues), see Phobius details amino acids 756 to 778 (23 residues), see Phobius details PF04945: YHS" amino acids 26 to 71 (46 residues), 54.3 bits, see alignment 3.3e-18 PF19335: HMBD" amino acids 85 to 111 (27 residues), 56.8 bits, see alignment (E = 4.9e-19) TIGR01511: copper-translocating P-type ATPase" amino acids 177 to 780 (604 residues), 597.8 bits, see alignment E=4.8e-183 TIGR01525: heavy metal translocating P-type ATPase" amino acids 196 to 780 (585 residues), 636.5 bits, see alignment E=9.4e-195 TIGR01512: cadmium-translocating P-type ATPase" amino acids 237 to 780 (544 residues), 404.7 bits, see alignment E=1.2e-124 TIGR01494: HAD ATPase, P-type, family IC" amino acids 241 to 753 (513 residues), 304.7 bits, see alignment E=1.9e-94 PF00122: E1-E2_ATPase" amino acids 270 to 450 (181 residues), 196.2 bits, see alignment E=1.1e-61 PF00702: Hydrolase" amino acids 468 to 685 (218 residues), 136.6 bits, see alignment E=4.3e-43 PF12710: HAD" amino acids 607 to 681 (75 residues), 28.8 bits, see alignment E=5e-10

Best Hits

Swiss-Prot: 69% identical to SILP_SALTM: Silver exporting P-type ATPase (silP) from Salmonella typhimurium

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 97% identity to sch:Sphch_4178)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (785 amino acids)

>GFF5207 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Sphingobium sp. HT1-2)
MNETHGAAHGGGCCGGHSAAKAATGVKDPVCGMTVDPATTAHQAEHGGERYHFCSAGCRT
KFIADPERYLGPPAPPVAAPEGTIWTCPMHPEIRQDHPGSCPICGMALEPATVTADSGPS
HELVDFTRRFRVGLMLALPVLILEMGAHLFPAIHRLVPMSISVWIQFVLATPVVLWAGWP
FFERGWASLKTRNLNMFTLIAMGTGVAWIYSVVATLAPQLFPPAFRGEDGMVAVYFEAAA
VITVLVLLGQMLELRARERTSGAIKALLNLAPKTARRIGSDGSEEEISLDLVAVGDRLRV
RPGEKVPVDGVVEDGRSSLDESMVTGESMPVTKAKADTVIGGTLNQTGALVIVADKVGRD
TMLARIVQMVAEAQRSRAPIQRMADQVSGWFVPVVIAVAVVAFIAWGIWGPEPRFAYGLV
AAVAVLIIACPCALGLATPMSIMVGVGRGAGLGVLIKNAEALEHMEKVDTLVVDKTGTLT
EGRPAVTQIVPAPGFDEAELLRLAASVERASEHPLALAIVEAAKDRGIPTSDVTDFDSPT
GRGALGTVDGRRIVLGNARFLSEEGIATDALAEQADALRRDGATAIFIGVDGTVGGAFAI
ADPVKQTTPEALAALKAEGIRVVMLTGDNRTTAEAVARRLGIDDVEAEVLPDQKSAVVAR
LKSEGRVVAMAGDGVNDAPALAAADVGIAMGSGTDVAIESAGVTLLKGDLMGIVRARRLS
QATMSNIRQNLVFAFIYNVAGVPVAAGALYPLFGILLSPIIAAAAMALSSVSVVTNALRL
NRKAL