Protein Info for GFF5206 in Sphingobium sp. HT1-2

Annotation: Methyltransferase Sare_0198

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 224 PF01209: Ubie_methyltran" amino acids 24 to 150 (127 residues), 49.7 bits, see alignment E=1.3e-16 PF13489: Methyltransf_23" amino acids 25 to 150 (126 residues), 58.2 bits, see alignment E=3.4e-19 PF05175: MTS" amino acids 32 to 143 (112 residues), 41.8 bits, see alignment E=3.7e-14 PF13847: Methyltransf_31" amino acids 40 to 148 (109 residues), 55.9 bits, see alignment E=1.6e-18 PF00891: Methyltransf_2" amino acids 43 to 145 (103 residues), 28.3 bits, see alignment E=4.2e-10 PF13649: Methyltransf_25" amino acids 45 to 139 (95 residues), 68.9 bits, see alignment E=2e-22 PF08242: Methyltransf_12" amino acids 46 to 141 (96 residues), 63.4 bits, see alignment E=1.1e-20 PF08241: Methyltransf_11" amino acids 46 to 143 (98 residues), 61.4 bits, see alignment E=4.4e-20

Best Hits

KEGG orthology group: None (inferred from 77% identity to sjp:SJA_C1-04840)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (224 amino acids)

>GFF5206 Methyltransferase Sare_0198 (Sphingobium sp. HT1-2)
MQSYTPPLGTGSTKDYDRAIRLWTREKRWRAAMLAALAPKAGETVVDVGCGTGSFALMLK
QAEPRTQVIGLDPDAEALDIARHKAAVRRADIDWRQGFARDVAPGTADAVVSSLVLHQMP
VREKAATLRAMFAMLRPGGRLVIADYGRQQGFMRLAFRLTVQRLDGIDDTRPNADGVLPG
LIAAAGFRHVAETFRLLTITGAIDLFTAHRPAQDHGLTVANDLG