Protein Info for GFF5205 in Variovorax sp. SCN45

Annotation: Cyanophycin synthase (EC 6.3.2.29)(EC 6.3.2.30)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 855 TIGR02068: cyanophycin synthetase" amino acids 2 to 852 (851 residues), 1172 bits, see alignment E=0 PF18921: Cyanophycin_syn" amino acids 37 to 123 (87 residues), 55.6 bits, see alignment E=1.2e-18 PF08443: RimK" amino acids 202 to 304 (103 residues), 24.3 bits, see alignment E=5.4e-09 PF02786: CPSase_L_D2" amino acids 204 to 287 (84 residues), 30.8 bits, see alignment E=5.2e-11 PF08245: Mur_ligase_M" amino acids 474 to 684 (211 residues), 72.9 bits, see alignment E=9.1e-24 PF02875: Mur_ligase_C" amino acids 711 to 836 (126 residues), 53.8 bits, see alignment E=9.2e-18

Best Hits

Swiss-Prot: 40% identical to CPHA_GEMHP: Cyanophycin synthetase (cphA) from Geminocystis herdmanii (strain PCC 6308)

KEGG orthology group: K03802, cyanophycin synthetase [EC: 6.-.-.-] (inferred from 77% identity to mpt:Mpe_A1023)

MetaCyc: 40% identical to cyanophycin synthase monomer (Geminocystis herdmanii PCC 6308)
Cyanophycin synthase (L-arginine-adding). [EC: 6.3.2.30]; 6.3.2.30 [EC: 6.3.2.30]; Cyanophycin synthase (L-aspartate-adding). [EC: 6.3.2.30, 6.3.2.29]; 6.3.2.29 [EC: 6.3.2.30, 6.3.2.29]

Predicted SEED Role

"Cyanophycin synthase (EC 6.3.2.29)(EC 6.3.2.30)" in subsystem Cyanophycin Metabolism (EC 6.3.2.29, EC 6.3.2.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.-.-.-, 6.3.2.29, 6.3.2.30

Use Curated BLAST to search for 6.-.-.- or 6.3.2.29 or 6.3.2.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (855 amino acids)

>GFF5205 Cyanophycin synthase (EC 6.3.2.29)(EC 6.3.2.30) (Variovorax sp. SCN45)
MKVTRIRALRGPNLWSRHTAIEAVVACHGDENAVSRLAGFEDRLRARFPTIGELHPMVLG
QPLALAHVLENAAVALQAQAGCAVNFGHTSPTTEEGVYQVVFQYTEEAVGRRAAELAEQL
IAAALADTPFDAEAAITELRDLDESERLGPSTGSIVDAAVARGIPYRRLTSGSLVQFGWG
AKQRRIQAAEVDSTSGVAESIAQDKELTKQLLNAAGVPVPLGRPVRDAEDGWAAALEIGL
PVVVKPQDGNQGKGVTVNINTREELFSAYDSAAAYGEVMVEKFLPGFDFRLLVVGDRLVA
AARRDPPQVIGDGSATVRQLIDTVNLDPRRGEGHATSLTKIRLDDIAIGRLESQGLTPDS
VPARGQRVVLRNNANLSTGGTATDVTDTVHPEVAARAVDAAQMVGLHICGVDMVCENVLR
PLEEQHGGVVEVNAAPGLRMHISPSFGRGRAVGEAVMDTMFAHGDDGRIPVVAVTGTNGK
TTTARLINHLLASSGLRTGMTNTDGVYVDGRQTDSGDCSGPKSARNVLMHPDVDAAVFEV
ARGGVLREGLGFDRCQVAVVTNIGSGDHLGLNYITTVEDLAVLKRVIVRNVSPDGYAVLN
AADVNVAAMATGCPGNVIFFTADRMHPVMATHRAQGKRTVYVDQDTLVAAEGSWRERIAL
RDVPITRGGTIGFQVDNVMASVAAAWAVGLDWDTIRSGLASFMNDAAGVPGRFNVMDYRG
ATVIADYGHNTDAMRALVSAVDTMPAKKRSVVISGAGDRRDSDIRDQTAILGEAFDDVIL
YQDAAQRGRADGEVMALLRQGLQGAARTRQIDEIRGEFVAIDAALARLQPGDLSLILVDQ
VEEALAHLAQRIAAG