Protein Info for GFF52 in Variovorax sp. SCN45

Annotation: Polyketide synthase modules and related proteins

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 208 to 553 (346 residues), 279.6 bits, E=1.9e-86
• TIGR01733: amino acid adenylation domain
  • amino acids 229 to 627 (399 residues), 402.1 bits, E=1.3e-124
• PF13193: AMP-binding_C
  • amino acids 611 to 685 (75 residues), 46.2 bits, see alignment (E = 3.5e-15)
• PF00550: PP-binding
  • amino acids 714 to 777 (64 residues), 45.8 bits, see alignment (E = 3e-15)
  • amino acids 1720 to 1786 (67 residues), 39.4 bits, see alignment (E = 2.9e-13)
• PF00109: ketoacyl-synt
  • amino acids 801 to 1050 (250 residues), 231.8 bits, E=5.2e-72
• PF02801: Ketoacyl-synt_C
  • amino acids 1058 to 1177 (120 residues), 132.2 bits, 4.9e-42
• PF16197: KAsynt_C_assoc
  • amino acids 1179 to 1291 (113 residues), 67.1 bits, 8.7e-22
• PF22621: CurL-like_PKS_C
  • amino acids 1240 to 1303 (64 residues), 66.2 bits, see alignment (E = 1e-21)
• PF22336: RhiE-like_linker
  • amino acids 1244 to 1305 (62 residues), 44.9 bits, see alignment (E = 4.5e-15)
• PF00698: Acyl_transf_1
  • amino acids 1326 to 1629 (304 residues), 171 bits, E=2.4e-53
• PF00202: Aminotran_3
  • amino acids 1961 to 2319 (359 residues), 162.1 bits, E=9e-51

Predicted SEED Role

"Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 2.3.1.39)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (48/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• fatty acid biosynthesis initiation (type II) (3/3 steps found)
• superpathway of fatty acid biosynthesis initiation (4/5 steps found)
• fatty acid biosynthesis initiation (mitochondria) (2/4 steps found)
• fatty acid biosynthesis initiation (plant mitochondria) (1/4 steps found)
• mycobactin biosynthesis (4/11 steps found)
• pederin biosynthesis (2/14 steps found)
• bryostatin biosynthesis (2/19 steps found)
• mupirocin biosynthesis (1/26 steps found)
• corallopyronin A biosynthesis (2/30 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.39

Use Curated BLAST to search for 2.3.1.39

Protein Sequence (2409 amino acids)

>GFF52 Polyketide synthase modules and related proteins (Variovorax sp. SCN45)
VSRANPETPSERHAISLALEPELLQRRAQQPATAAQLPDTLFAAAWLLLQSRWLGVLWPV
LHERVGAQALPVSTTVAFDATRPAGEWLAELDAARRAAAHTAAGAEPPSSLWLREATGTA
TPDARLLLWLDEASASLHVDAAAGLMDMASAEHLLAALADTAADLLARPDAALNDIRTVP
GTGRDDQLLRWNTPASRLDTALTVTGLFRRQVNAAPDAIALVEGDARLSYAELDRRCDLI
ARRLQQLGVRSGDSVGLLLDRSLAAVVALIGILKAGGAYVPVPTDFPPERIAYMFGEAQA
RHVVTTQAFRHLVPAEQAVFLLDDTLDNAERGSWNEPAIDGESVAYVMYTSGSTGTPKGI
EICHRSILRLVVGVDYVDLAPGRAMLHAAPLGFDAATLEIWGPLLNGGCCVVHDERVPTG
AGLARTIARHGVHTAWLTAALFNAVVDDDPTHLAGLSQLFTGGEALSVPHVRRALAALPG
LTLSNGYGPTECTTFATTHRIAPTLPADTRSVPLGRPIKDTVLRVLSPAMALLPCGFVGE
LCIGGHGLARGYLRQPELSADRFVPDPFGGPDDRLYRTGDLARWLPDGTIEFIGRRDGQV
KIHGHRIETGEIEAAILAHPEVQSCAVVARPDADGQLRLVAYLVARGRQLSWQALRSHLA
ARLPAALMPAAQVWLAQLPVTPNGKLDRRALPEPAAGRPELSQPYEEPQDAVEQQVCEAF
ARALRIDKVGRNDNFFDLGGDSLLVLQVLAELKRDTALPLSTNLFFRDPTPKAMAARMRP
PVEAPVQPAPSAPRPAAPASEAVALIATAGRFPGAADVEQFWDNLVAGRDTVSFFDDATL
DAGVSEALRRDPAYVRARGVIDGIENFDAAFFGIGPKEAQLMDPQQRVFLEICWECLERA
GYVPDAAPGPVGVYAGMYNATYFQRHVSTRPDLVEPVGEFQVMLANEKDYITTRVANRLN
LTGPAVSVHTACSTSLVAVAHAFHALRTGQCYMALAGGASVTCPPRSGYLYNEGSMLSPD
GHTRSFDAKAQGTVFSDGAAVVLLKRLADAQADGDTIYAVLRSACVNNDGGAKASFTAPS
VDGQAAVIRAALAAAEVDARSISYVEAHGTATPMGDPIEVEALAVAYAEHTDALDYCTLG
SLKSNVGHMVTAAGAAGLIKTALALHHAQIPPTVHFEAPNPAIDFARTPFRVSASLQPWP
RGDLPRRAGVSSFGVGGTNAHVIVEEAPERPASPLATGEQMLPLSARSEAALDIATEQLA
AHLQAHADQPLADVAHTLAVGRKAHAFRRVIVAADTAQAVAALRTADSPWRASGRLASRA
PQPVLMFPGQGAQYAGMGRLLHAADPVFAAAFDDCIDAVVGTTDFDLRERMFSDDPKALS
PTAVTQPALFTIEYALARRLLATGVRPHALMGHSVGEFVAAVLAGVMRLEDAARLVARRG
ALMQALPAGAMLSVRIGAAELMEKLGPSLSLAAENGPTACVAAGPFEAIAALQAALEAEG
IACRTLQTSHAFHSAMMDGAVAPFEALVSQVALHAPQTPIFSTLTGMLLEAEEATSPAYW
ARHLRGAVRFSPAVRAAMAHAAQPLFIEAGPRNTLSTLVRQHGASTAVPLLHGELADEDR
TLRLAVGRLWTCGADVELSRLSSRVGAQRVCLPTSPFERKRFWVDIAAMPAIAAIPETPV
APLSASPAPSATPTPLEPIVTAAATSPLPPSASSPPVDARLRALFEDISGIDMAQAEGHA
PFGELGLDSLTLTQAATQIKKHFKVNLSFRQLMENYRCFDALSAFLRESLPPEAAPVAAA
APAAEAPAVLQPVMPAPLVHAPMQGNVASSPISQLIAQQMELMRQQLALLSGAAPAAPVM
LQAMPAPAVAAPVATPAAEEPVPSKEPLRYDVTKAFGAIARIHTQRTAEPSGRQKARLAA
FMRRYIERTLKSKQFTEANRPHMADPRVVNGFRPVTKEITYQIVIERSKGSKMWDLDGNE
YVDALNGFGMNMFGWQPDFVQEAVRKQLDDGYEIGPQHPLAAEVTALICELTGCDRAGLC
NTGSEAVMAALRIARTVTGRSTVVVFTGSYHGTFDEVLVRAGKGGKGLSAAPGVMSGMFG
DIRVLDYGTPEALAFIRENADDLAAVLAEPVQSRRPDFQPREFLHEVREITARSGCALIF
DEVITGFRTALGGAQELFGVRADLATYGKVIGGGFPVGVIAGKREFMDALDGGPWQYGDD
SIPGVGVTYFAGTFVRHPLALAAAKASLTHLKQAGPALQAGLTSSTGAMASELSAWCKEV
GAPIEIRHFGSLWRVSWLEDHPLQDLLFAMMRSRGVHILDNFPCFLTSAHSAEDIAFIQR
AFKESVAEMQESGFLPRRAAVVTSFDTRRPTEEGSVLARDVDGQPFWYVPEAQSSAQASG
HLVNGKAAA