Protein Info for GFF519 in Sphingobium sp. HT1-2

Annotation: Acetate permease ActP (cation/acetate symporter)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 566 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 32 to 51 (20 residues), see Phobius details amino acids 78 to 98 (21 residues), see Phobius details amino acids 104 to 123 (20 residues), see Phobius details amino acids 149 to 170 (22 residues), see Phobius details amino acids 180 to 199 (20 residues), see Phobius details amino acids 211 to 233 (23 residues), see Phobius details amino acids 280 to 302 (23 residues), see Phobius details amino acids 314 to 339 (26 residues), see Phobius details amino acids 372 to 399 (28 residues), see Phobius details amino acids 421 to 440 (20 residues), see Phobius details amino acids 446 to 470 (25 residues), see Phobius details amino acids 478 to 500 (23 residues), see Phobius details amino acids 512 to 531 (20 residues), see Phobius details TIGR00813: transporter, solute:sodium symporter (SSS) family" amino acids 64 to 485 (422 residues), 201.5 bits, see alignment E=1.2e-63 PF00474: SSF" amino acids 64 to 485 (422 residues), 384.2 bits, see alignment E=3.6e-119

Best Hits

KEGG orthology group: K14393, cation/acetate symporter (inferred from 92% identity to sjp:SJA_C1-07850)

Predicted SEED Role

"Acetate permease ActP (cation/acetate symporter)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (566 amino acids)

>GFF519 Acetate permease ActP (cation/acetate symporter) (Sphingobium sp. HT1-2)
MRARLAGALAIMLLPTSAFAAALEGEAQRQPINWVAIAMFVAFVGLTLWITKAAAARTRT
ASDFYTAGGGISGFQNGLAMAGDYMSAASFLGISAQIFTDGYDGLIYSTGFLVGWPILLF
LMAERLRNLGRFTFADVASYRFAQPPVRSFAAVSTLLVVSFYLIAQMVGAGQLIKLLFGL
PYAVAVVIVGALMMLYVLFGGMRATTWVQIIKAVLLLGGASFMALMVLAQFGFSLEALFA
RAVAVKSAPAQAAGATPAEAAAKGRAIMAPGGFIKDPVSAISFGLALMLGTAGLPHILMR
FFTVPDAKEARKSVLWATGWIGYFYILTFIIGFGAIILVGTDAGYKMADGTLRGGGNMAA
IHLAHAVGGNAFLGFISAVAFATILAVVAGLTLSAASAVSHDLYATVLKQGRANSADELR
VSRITTLALGALAVLLGLVFEKQNVAFMVSLAFALAASGNFPVLILSLLWSGCTTHGAAW
GGTIGLLTAFVLTLLSPAVWTTTFGLGPAPFPYSSAAIFSVPAGFIAIWLISRLDRSPRA
AVDKAGYPAQRVRAETGIGAFAASDH