Protein Info for GFF5185 in Variovorax sp. SCN45
Annotation: FIG00732904: hypothetical protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 72% identity to vpe:Varpa_1106)Predicted SEED Role
"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)
MetaCyc Pathways
- peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
- peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
- peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
- peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
- peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.2.10
Use Curated BLAST to search for 6.3.2.10
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (1586 amino acids)
>GFF5185 FIG00732904: hypothetical protein (Variovorax sp. SCN45) MTIDPNPLVDLLTRARQALADPAGMLPAANPATPEAGCVVFFATGEDSSRARVASGRGAN IEAAWQAAAEAIAAQAARGENPPQRLRAEVVDKVTPMRWRELKAKLAQTKRNYFNEGIAF DARFEIALLAQEMHGGAVLYNGDVDHAEPNPGNLHLLSKRRFGREIDFPSDDEAPVWCFT TRAVYVDANGAWPITAEGQAAGFRTLSDWNAPQIRTLIEDASTYLAGQVKPTGEFDYGWF PCFDRLIPTYNTLRHASSTYAMLEAWELTRSDTLKASIDRSIGFLAGTLIRPAVLPDGTR AAYLLDIGNEIKLGGSAVCLLALVKYMELTGDRQFLPLLEQLALGIRHMQDAETGSFVHV LTAPSLDVKDKFRVIYYDGEAAFGLMRLYGLTRDARWLAVVEKAFEHFIAAEHWRAHDHW LSYCVNELTRYRPETRYYQFGLRNVAGYLDFVLERITTFPTLLELMMAARDMVLRLEADP ALRPLLAELDRDKFDRALAYRARYLANGHFWPELAMFFRNPARIAGSFFIKHHSFRVRID DVEHYLSGYVAYHRMLKASEGQQQGRRAGTGTVAWGGDVNLGRRQHYRMHELGGARRMLG DIAALRDADLRIVNLECVVATTGEQGIDKGEAGPYYYRARPEMLAVLTEAGIGMVATANN HSGDYGAEALLEQQTHLDAAGIAHAGTGRTLEAAWRPAFRRAGGVRVALFSLDATQQRFA ASAERAGAAWLPLDDPAAWTAELAPRIEAARREADVVLVAVHWGANLETAPSAAEVAVGH AIVDAGADAVLGTSAHVLQGIEVYRGRPILHDAGDLLFDSIRNDFGDSGVFTLALGLHGV EAVVFTPVGVGYGFSRQLAGEQAEALSARFAEQCRALGTQMTMVQGGRCALRLSPPERPA FQADPASAVAPAVRPAPVVGPVRAPRPEWLADAVPDDARITPLQIGPLRLLGLRIAPRQI VGRRMLWVESFWCIDEPVAADLRLQFRALPMRSSTMPPWGEGMDHDPCDWMWPTSRWEPG RIYRDRYGLRPPRPGLLESDVLQLELRLRGNLPELPPELSSARRLQVWCGLRVPDFPVPL PERPAPVLAVDRLGEGNAPAPVWTADELQQITGGRWMVEPPPGWFVNSVVRGPLHMSMLP APSLFVASDYRTLAVHESYSKPRADNWDRHLELPGLQHALAGAVVAHEVEGLDAAFPLLQ VQDPIAAMLALGAAARARYKGLLVGVTGTVGKSSTIAMLRDLLPERARVHTTIDNYNSRV GVPAMLANLAADTDVCILEMAQSGLWMERGPISLKARPHVAVITEIGLSQTRQASTLELT AEYKSRIFLGLEPGGVAIVPDHIPCLQQVVQAAARTAGAIWVVGTGPEANIRIVDTQPEP GGGCRVRIAMAGRTVAYSFPLHSAGLVRNSALAFAALVAMGFDAEDACARMPHVRLPASV MQVHPLRTQAGVQATLIDDSWNAEVMSMRNAMEFVRGFQRAAQGPVVRKIGVLGRIVNLD KDAEAMHRSLAAPLLQSGIAHVVTHGDEMRWLREEVPAELLGPHFDDASKLAGYLGGFLR DGDLVLVKGDRIGSDFGEVPVLLRKL