Protein Info for PS417_02635 in Pseudomonas simiae WCS417

Annotation: nicotinate phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01514: nicotinate phosphoribosyltransferase
  • amino acids 10 to 398 (389 residues), 497.2 bits, E=1.8e-153
• PF17767: NAPRTase_N
  • amino acids 15 to 132 (118 residues), 118.3 bits, E=2.8e-38
• PF04095: NAPRTase
  • amino acids 172 to 398 (227 residues), 297 bits, E=1.2e-92

Best Hits

Swiss-Prot: 93% identical to PNCB_PSEPF: Nicotinate phosphoribosyltransferase (pncB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00763, nicotinate phosphoribosyltransferase [EC: 2.4.2.11] (inferred from 100% identity to pfs:PFLU0547)

MetaCyc: 53% identical to nicotinate phosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
NICOTINATEPRIBOSYLTRANS-RXN [EC: 6.3.4.21]

Predicted SEED Role

"Nicotinate phosphoribosyltransferase (EC 2.4.2.11)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation or Redox-dependent regulation of nucleus processes (EC 2.4.2.11)

MetaCyc Pathways

• NAD salvage pathway I (PNC VI cycle) (5/7 steps found)
• NAD salvage pathway V (PNC V cycle) (3/5 steps found)
• superpathway of NAD biosynthesis in eukaryotes (9/14 steps found)
• NAD salvage (plants) (5/11 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Nicotinate and nicotinamide metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.11, 6.3.4.21

Use Curated BLAST to search for 2.4.2.11 or 6.3.4.21

Sequence Analysis Tools

See A0A1N7U1E9 at UniProt or InterPro

Protein Sequence (404 amino acids)

>PS417_02635 nicotinate phosphoribosyltransferase (Pseudomonas simiae WCS417)
MSESVFADRIVQNLLDTDFYKLTMMQAVLHNYPNVEVEWEFRCRNSEDLRPYLAEIRYQI
ERLAELSLSPDQLGFLERISFMKPDFLRFLGLFRFNLRYVQTGIENGELFIRLRGPWLHV
ILFEVPMLAIVSEVRNRYRYQTVVLEQAREQLYRKFDWLTANASSDELSELQVADFGTRR
RFSYRVQEEVVSVLKHDFPGRFVGTSNVHLARELDMKPLGTMAHEWIMAHQQLGPRLIDS
QIAALDCWVREYRGLLGIALTDCITTDAFLGDFDLYFAKLFDGLRHDSGDPVQWAEKAIA
HYHKLGIEPMSKTLVFSDSLSLPKALEIFRALRGRINVSFGIGTNLTCDIPGVEPMSIVL
KMTACNGQPVAKISDEAGKTHCTDPNFVAYLRHVFKVPAISSKE