Protein Info for PS417_26365 in Pseudomonas simiae WCS417

Annotation: EmrB/QacA subfamily drug resistance transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 475 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 53 to 73 (21 residues), see Phobius details amino acids 81 to 103 (23 residues), see Phobius details amino acids 110 to 127 (18 residues), see Phobius details amino acids 139 to 160 (22 residues), see Phobius details amino acids 167 to 188 (22 residues), see Phobius details amino acids 200 to 219 (20 residues), see Phobius details amino acids 230 to 248 (19 residues), see Phobius details amino acids 268 to 292 (25 residues), see Phobius details amino acids 304 to 322 (19 residues), see Phobius details amino acids 330 to 352 (23 residues), see Phobius details amino acids 358 to 378 (21 residues), see Phobius details amino acids 398 to 421 (24 residues), see Phobius details amino acids 434 to 456 (23 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 18 to 418 (401 residues), 239.2 bits, see alignment E=4.2e-75 PF07690: MFS_1" amino acids 19 to 197 (179 residues), 111.3 bits, see alignment E=5e-36 amino acids 273 to 467 (195 residues), 42 bits, see alignment E=6.1e-15 PF00083: Sugar_tr" amino acids 47 to 115 (69 residues), 28 bits, see alignment E=1.1e-10

Best Hits

KEGG orthology group: None (inferred from 97% identity to pfs:PFLU5690)

Predicted SEED Role

"Permeases of the major facilitator superfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U2J7 at UniProt or InterPro

Protein Sequence (475 amino acids)

>PS417_26365 EmrB/QacA subfamily drug resistance transporter (Pseudomonas simiae WCS417)
MPNRVPLDAKTARWLPWVVAIAFFMQSLDGTILNTALPAMARDLAENPLRMQGVVIAYML
TVALLIPASGWIADRFGTKKIFFGAIMLFSIGSLLCALSSSLTMLVGARVIQGLGGALML
PVGRLVVLRAYPRSELVRIMGFITIPGLLGPLLGPTMGGWMVQYLTWHWIFLINLPVGMI
GCYAVWKFIPDLRGTERTRFDGIGFLLFGAAMILITIAMEGLGELHLPHLRVMLLLFGGL
ACLAAYWLRAGHIDNPLFSPVLFKTRTFAVGILGNLFARLGSGALPFLVPLLLQVALGYS
PSEAGMSMLPLAAAAMFAKSVARPLIEHLGYRIVLTGNTLALGIMLASMGLVTEHTPYPL
LLGMLAVLGAINSLQFTAMNTVTLIDLDDAQASSGNSLLSVVAQLSLSLGVACAGALLGG
FTAETGNDGVESVLGAFQLTFLTVGIMAMLAAAIFLQLSPKDGKRAASPEQHIEH