Protein Info for GFF5141 in Sphingobium sp. HT1-2

Annotation: Periplasmic aromatic aldehyde oxidoreductase, molybdenum binding subunit YagR @ 4-hydroxybenzoyl-CoA reductase, alpha subunit (EC 1.3.99.20)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01315: Ald_Xan_dh_C
  • amino acids 62 to 174 (113 residues), 86.6 bits, E=2.2e-28
• PF02738: MoCoBD_1
  • amino acids 201 to 423 (223 residues), 180.4 bits, E=5.1e-57
• PF20256: MoCoBD_2
  • amino acids 448 to 721 (274 residues), 175.1 bits, E=3.5e-55

Best Hits

KEGG orthology group: K11177, xanthine dehydrogenase YagR molybdenum-binding subunit [EC: 1.17.1.4] (inferred from 76% identity to rpc:RPC_4640)

Predicted SEED Role

"Periplasmic aromatic aldehyde oxidoreductase, molybdenum binding subunit YagR"

MetaCyc Pathways

• adenosine nucleotides degradation I (7/8 steps found)
• adenosine nucleotides degradation II (4/5 steps found)
• purine nucleotides degradation I (plants) (9/12 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• guanosine nucleotides degradation II (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• superpathway of guanosine nucleotides degradation (plants) (4/6 steps found)
• guanosine nucleotides degradation III (2/4 steps found)
• purine nucleotides degradation II (aerobic) (7/11 steps found)
• ureide biosynthesis (3/7 steps found)
• purine nucleobases degradation II (anaerobic) (15/24 steps found)
• superpathway of purines degradation in plants (9/18 steps found)
• caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
• theophylline degradation (1/9 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4

Use Curated BLAST to search for 1.17.1.4

Protein Sequence (777 amino acids)

>GFF5141 Periplasmic aromatic aldehyde oxidoreductase, molybdenum binding subunit YagR @ 4-hydroxybenzoyl-CoA reductase, alpha subunit (EC 1.3.99.20) (Sphingobium sp. HT1-2)
MSLIDSGKALAQGLMQSAMAKVVALAPDSWMPGGEPDPLIRHQHGHVGKPVSRIDGPLKV
SGAALFAAEFPLEDMVYAAIVYSKIAKGRITSLDVQAAEKAPGVVLVMTYRNAPRLKPAP
LFMSDPKAAAGDSIPVLQDEKIYWNGQPIAIVLADTQEQADHAAALVDVKYAAEPAETDF
HAAIAKGTTQSQFMGQPLHDEVGDAQGAFEKAAVKIDAAYSTPRHNHNPIELHAVTLSWQ
GDMLRIHDAQQAVAHSAWTLGQVFDLPADKVVITSPYVGGGFGSKTLWQHQIIAAAAAKL
AERPVRLMLSREGVYRIVGGRSPTEQRVALGSDAQGKLTALIHRGVTAKTSHNVMPEPFI
LPTRSAYAAENILLDVQHVEIDMTSNTFMRAPGEAVGTFALECAMDELAEKLGIDPIELR
LRNEPEKDPISGLPFSARHIDQAWRDGAERFGWSARNPMPRMQQEGEWLVGMGCATATYP
YYRMPGGAARITLSRDGQAMIDIAAHEMGMGTATAQTQVAAERLGLPMEQIRFNYGDSTL
PGLVLAGGSQQTASIGASVIAAYNKLVVELLALAGNDSPLAGLKHSEVGSRDGGLASLED
PSRYESYASILSRAQRDAVVIEAEPPMPLETMHWSMHSHGAMFCEVRVNMVTGEPRVSRF
LGSFDCGRILNPKTAASQFRGGIIMGLGLALMEEAQFDERSGRIMNPSLAEYHVPVHMDV
PEIDVIWTDVPDPHTPMGARGVGEIGITGTGAAVANAIYNATGRRVRDLPITLEKLI