Protein Info for PS417_26330 in Pseudomonas simiae WCS417

Annotation: beta-aspartyl peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 663 transmembrane" amino acids 20 to 38 (19 residues), see Phobius details amino acids 58 to 78 (21 residues), see Phobius details amino acids 97 to 117 (21 residues), see Phobius details amino acids 149 to 169 (21 residues), see Phobius details amino acids 197 to 217 (21 residues), see Phobius details amino acids 237 to 257 (21 residues), see Phobius details amino acids 269 to 289 (21 residues), see Phobius details amino acids 328 to 346 (19 residues), see Phobius details amino acids 358 to 377 (20 residues), see Phobius details amino acids 417 to 438 (22 residues), see Phobius details amino acids 458 to 478 (21 residues), see Phobius details amino acids 484 to 505 (22 residues), see Phobius details PF02028: BCCT" amino acids 20 to 512 (493 residues), 575.3 bits, see alignment E=4.6e-177 TIGR00842: transporter, betaine/carnitine/choline transporter (BCCT) family" amino acids 58 to 512 (455 residues), 503.9 bits, see alignment E=1.9e-155

Best Hits

KEGG orthology group: K02168, high-affinity choline transport protein (inferred from 99% identity to pfs:PFLU5683)

Predicted SEED Role

"High-affinity choline uptake protein BetT" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UPX0 at UniProt or InterPro

Protein Sequence (663 amino acids)

>PS417_26330 beta-aspartyl peptidase (Pseudomonas simiae WCS417)
MSSASLIKTPPEKVRVNGWVFYTSTALILLLTAILIIAPQEAGRMLGVAQAWLSKSFGWY
YMVVIAAYLVFVVGLAFSSYGKLKLGSKDDTPDFSYGAWAGMLFSSGIGISLLYFGASEP
LDHYFNPPEGAAASNGAARQALQLTFLHWGLHGWAIYALVGLAVAYFAYRHNQPLALRSA
LYPLVGERWVKGAAGHAVDGFGMFVTLLGLVTNLGIGSMQVSSGLENLFGMEHSNTNLLI
VIIVMSTVATIAAVSGVENGIRRLSNLNIVLFSGLLIFVLLFGPTLHLLNGLVQNTGDYL
NGIILKTFDLYVYEGDADKTERWMGLWTLFYWAWWISWAPFVGMFIARISRGRTVRELVA
GVLLIPLGFTLAWLSIFGNSALDLVLNQGAVALGKTALEQPSMAIYQLLEHYPASKIVIG
VSIFVGFVLFLTPADSGAVMMANLSCKGGNVDEDAPHWLRIFWSAVITLVTIGLLFAGNF
EAMQTMVVLAGLPFSVVLIFFMFGLHKAMRQDVAIEQEQAQLAERGRRGFSERLTALDLQ
PSQGTVQRFMDKHVTPALEEAATALREQGLEVQTLLGKSKRCIGVRIEMAEGNPFVYEVS
LDAYSSAPSDLPEEERTRYYRAEVYLHNGPQEYDLMGFTQEQITRDVLDQFESHRQLLGR
VYS