Protein Info for GFF5140 in Sphingobium sp. HT1-2

Annotation: Phytochrome, two-component sensor histidine kinase (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 863 PF08446: PAS_2" amino acids 25 to 131 (107 residues), 50 bits, see alignment E=1.4e-16 PF01590: GAF" amino acids 155 to 319 (165 residues), 54.4 bits, see alignment E=6.1e-18 PF00360: PHY" amino acids 335 to 512 (178 residues), 155.1 bits, see alignment E=4.1e-49 PF07568: HisKA_2" amino acids 534 to 583 (50 residues), 25.9 bits, see alignment 2.5e-09 PF07536: HWE_HK" amino acids 534 to 614 (81 residues), 67.1 bits, see alignment E=5.9e-22 PF00072: Response_reg" amino acids 753 to 858 (106 residues), 31.1 bits, see alignment E=7.1e-11

Best Hits

KEGG orthology group: None (inferred from 68% identity to hdn:Hden_0770)

Predicted SEED Role

"Phytochrome, two-component sensor histidine kinase (EC 2.7.3.-)" in subsystem Oxidative stress or Oxygen and light sensor PpaA-PpsR (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (863 amino acids)

>GFF5140 Phytochrome, two-component sensor histidine kinase (EC 2.7.3.-) (Sphingobium sp. HT1-2)
MPDLCTFKDLGDSVADFDKTDLTNCDREPIHLLGAIQQPGFLIAVSTDWIIARVSANIRD
LLGSDPEALIGAPLPDYVPATFLHDLRNRISLLAEPDMVERLFACQIGTSGQPYDVAVHF
SNGQIVIEAEPGSAAGGDATNAVRGMMSRLDRTKDMTAFFREGARQVRALLGYDRVMVYK
FAASGAGAVVAEASKTGIGSFLGLNYPASDIPRQARELYKRNLVRVIRDVDAQPVPVVPE
IDRSGKRLDLSLSILRSVSPIHIEYLKNMGVAASLSISIVVDDKLWGLFACHHYSPRSPT
FERRSVCELFAEMFAMRLESRERRQVAEYEERARNISDQLLGAVASDETLLQDPDWLGDI
LTSAIPADGVGVWINGNYAFSGVTPPTEEFRRIVRALNGLAAGRVFTTDTIGSLVDGADE
YAEQAAGLLAIPISRSPRDYVMLFRKELKQSVRWAGDPHKPIEYGPNGPRLTPRESFAEW
KETVEGHAEPFTESELRVAETLRATLIEVVLRLADEAAAERRQSTARQEMLIAELNHRVR
NILGVIRGLIRQSQPDSPEVKEFVRLVDGRIHALARAHNQITDDHWGPAPLGALIDAEAA
AFAADRQSAIIANGTPTLINPQAYSTMALVVHELVTNSTKYGSLSGTGEVHIDWSRDHKG
DLRFVWQESGGPTVQAPKRKGFGTTIIKRSVPYDLGGQANIEYAPEGVRAEFLIPSRHVS
DARKVDGPTIHIQRSNVSSQHPVDIGVAGQKSVLLVEDSLIIALDAEDILNRLGASVSTA
SIAEAAHDLLDEARFDFAILDINLGDQTSLGIADRLQEQGVPYFFASGYGEQASLPMEHR
SAMVVQKPYTTHNVAKAVQELFA