Protein Info for PS417_26295 in Pseudomonas simiae WCS417

Annotation: gamma-butyrobetaine dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 386 PF06155: GBBH-like_N" amino acids 27 to 104 (78 residues), 50.6 bits, see alignment E=2.7e-17 TIGR02409: gamma-butyrobetaine hydroxylase" amino acids 29 to 386 (358 residues), 434 bits, see alignment E=2.3e-134 PF02668: TauD" amino acids 134 to 371 (238 residues), 156.2 bits, see alignment E=1.7e-49

Best Hits

Swiss-Prot: 67% identical to BODG_PSESK: Gamma-butyrobetaine dioxygenase from Pseudomonas sp. (strain AK-1)

KEGG orthology group: K00471, gamma-butyrobetaine dioxygenase [EC: 1.14.11.1] (inferred from 80% identity to pfl:PFL_5756)

MetaCyc: 67% identical to gamma-butyrobetaine hydroxylase subunit (Pseudomonas sp. AK-1)
Gamma-butyrobetaine dioxygenase. [EC: 1.14.11.1]

Predicted SEED Role

"Gamma-butyrobetaine,2-oxoglutarate dioxygenase (EC 1.14.11.1)" (EC 1.14.11.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1UYV5 at UniProt or InterPro

Protein Sequence (386 amino acids)

>PS417_26295 gamma-butyrobetaine dioxygenase (Pseudomonas simiae WCS417)
MQTAAAVADFRTYPKICDIADVRVLEDQIFVQWADGRVSPFHHQWLRDNCPCSQCVYSVT
REQVLEIVDVDAHLRPASARIDQGNLSLEWSGGHRSQYDPGWLRAHAYDDESRAERRAAT
PKSRLWDSRFQLPVFEYSAVMEDPSALLQWLLALRDSGLTQVRGVPTERDSLALVAKRIS
FIRESNFGVLFNVQSKADADSNAYTAFNLPLHSDLPTRELQPGLQFLHCLVNDANGGESI
FVDGFAIANALRTEDPEAFRALCEIPVEFRNKDRHSDYRRLAPIIALDALGDVAEIRMAN
FLRGPFETSVAQMPLLYRAYRRFIAMTREDRFRLVKRLNPGELWCFDNRRTLHARNAFDP
ASGARHFQGCYIDRDELLSRIRVLQR