Protein Info for PS417_26180 in Pseudomonas simiae WCS417

Annotation: sarcosine oxidase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1005 TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 2 to 1004 (1003 residues), 1546.9 bits, see alignment E=0 PF13510: Fer2_4" amino acids 17 to 102 (86 residues), 81.6 bits, see alignment E=1.9e-26 PF01134: GIDA" amino acids 172 to 201 (30 residues), 23.4 bits, see alignment (E = 1.6e-08) PF00890: FAD_binding_2" amino acids 172 to 209 (38 residues), 33.3 bits, see alignment (E = 1.8e-11) PF07992: Pyr_redox_2" amino acids 172 to 437 (266 residues), 45.6 bits, see alignment E=3.6e-15 PF03486: HI0933_like" amino acids 172 to 207 (36 residues), 26.6 bits, see alignment (E = 1.4e-09) PF12831: FAD_oxidored" amino acids 172 to 209 (38 residues), 39.8 bits, see alignment (E = 2.1e-13) PF13450: NAD_binding_8" amino acids 175 to 226 (52 residues), 27.6 bits, see alignment 1.6e-09 PF17806: SO_alpha_A3" amino acids 522 to 605 (84 residues), 107 bits, see alignment E=2.8e-34 PF01571: GCV_T" amino acids 620 to 885 (266 residues), 245.3 bits, see alignment E=3.3e-76 PF08669: GCV_T_C" amino acids 911 to 997 (87 residues), 48.5 bits, see alignment E=3.7e-16

Best Hits

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 94% identity to pfo:Pfl01_5205)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1UVG7 at UniProt or InterPro

Protein Sequence (1005 amino acids)

>PS417_26180 sarcosine oxidase subunit alpha (Pseudomonas simiae WCS417)
MSQTNRLSNGGRIDRNKVLTFTFNGQSYKGFEGDTLAAALLANGVDIIGRSFKYSRPRGI
FAAGAEEPNAVLQIGATEATQIPNVRATQQALYQGLVATSTNGWPNVNNDVMGILGKVGG
KLMPPGFYYKTFMYPQSFWMTYEKYIRKAAGLGRSPTENDPDTYDNFNRHCDVLVVGAGP
AGLAAALAAARSGARVILADEQEEFGGSLLDSRESLDGKPAAEWVASVIAELKALPDVVL
LPRATVNGYHDHNFLTIHERLTDHLGDRAPIGVVRQRIHRVRAKRVVLATGACERPLVYG
NNDVPGNMLAGAVSTYVRRYGVAPGKKLVLSTNNDHAYRVALDWLDAGLQVVAVADVRHN
PRGALVEEARAKGIRILTGSAVIEARGSKHVTAARVAAIDVKAHKVTSPGEWLDCDLVAS
SGGYSPVVHLASHLGGKPIWREDILGFVPGEAPQKRVCVGGINGVYGLGDSLADGFEGGV
RAASEAGFAPVEGTLPKALSRLEEPTLAVYQVPHDKPTARAPKQFVDLQNDVTAAAIELA
TREGFESVEHVKRYTALGFGTDQGKLGNVNGLAIAARSLNVTIPQMGTTMFRPNYTPVTF
GAVAGRHCGHIFEPVRYTALHAWHVKNGAEFEDVGQWKRPWYFPRNGEDLHAAVKRECLA
VRDSVGLLDASTLGKIDIQGPDAREFLNRIYTNAWTKLDVGKARYGLMCKEDGMVFDDGV
TACLADNHFLMTTTTGGAARVLQWLEIYQQTEWPDLKVYFTSVTDHWATMTLSGPNSRKL
LAEVTDIDLDKDGFPFMTWKEGLVGGVPARVFRISFTGELSYEVNVQADYAMGVLEKIVE
AGKKYNLTPYGTETMHVLRAEKGFIIVGQDTDGSMTPDDLNMGWCVGRTKPFSWIGWRGM
NREDCVREERKQLVGLKPIDPTVWLPEGAQLVFDPKQSIPMKMVGHVTSSYAHNSLGYSF
AMAVVKGGLKRIGERVFSPQADGSVIEAEIVSSVFFDPKGDRQNI