Protein Info for GFF5109 in Sphingobium sp. HT1-2

Annotation: Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.5.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 766 transmembrane" amino acids 7 to 27 (21 residues), see Phobius details amino acids 33 to 50 (18 residues), see Phobius details amino acids 58 to 80 (23 residues), see Phobius details amino acids 86 to 105 (20 residues), see Phobius details amino acids 114 to 132 (19 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 7 to 762 (756 residues), 948.6 bits, see alignment E=1.1e-289 PF01011: PQQ" amino acids 157 to 740 (584 residues), 719.2 bits, see alignment E=4.6e-220 PF13360: PQQ_2" amino acids 216 to 490 (275 residues), 31.7 bits, see alignment E=1.3e-11 amino acids 640 to 728 (89 residues), 25.4 bits, see alignment E=1.1e-09

Best Hits

KEGG orthology group: K05358, quinate dehydrogenase (quinone) [EC: 1.1.5.8] (inferred from 61% identity to rlt:Rleg2_3145)

Predicted SEED Role

"Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25)" in subsystem Chorismate Synthesis or Quinate degradation (EC 1.1.99.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.8, 1.1.99.25

Use Curated BLAST to search for 1.1.5.8 or 1.1.99.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (766 amino acids)

>GFF5109 Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.5.8) (Sphingobium sp. HT1-2)
MSKIAKLYAFILTIIGLALAASGLWLIALGGSLYYTIFGVALAASAILAIKGRREANWIL
AAALAGTVIWSIVEAGFRFWDLFPRLLAPIALVGLGFALFPSIITGAARKGFRLSAAACG
VAFIVLFGLAFVPHPTVSAIAAADYSPGAGNNAPQDWTSYGATTAGLRYATYNKINRDNV
TTLKPAWTIRTGDKGPGIDQNTPLQIGDTLYSCTRNNIIVALDPDTGATKWRFDPHAKAP
FWQRCRSLGFYQSASMTGECQQRLIATTIDARLMALDARTGKLCPGFGNGGTVSLAEGMG
PVKPGFYFQTSAPLVARNIIVIGGWVVDNEQTGEPSGVIRGFDVMTGKLVWAWDLGNPAI
TREPPPGQTYTRGTPNMWTTAAYDDKLGLIYVPLGNATPDYYGVQRKPWADQYNSTLVAL
DVTSGRERWKFQTVHHDLWDYDLPAQPALVNLRGNIPALLLSTKRGQLFLLNRATGQPLS
RVVEKPVSSAGSVPGERMSPTQPYSVDMPVIAETLNERRMWGMTMFDQLACRISFRQSRY
SGDFTPPGMTLSIEHPSNLGGLNWGSMSVDVPNNRVFMNDVRVPMTVRLMTRPEFTEFAK
KNHPDGTGHGPSPQLGTPYGVNIQMWMTKLGVPCSRPPFGTITAIDLNTRKIAWQVPAGT
AEELGPLGIKSHLPMPIGMPTYAGTSVTAGGLLFFAGSQDYYLRAYDTSNGKEVWRYRLP
VGSSATPMTYISPKTGRQYVLVSVGGAAHSKDIGDYVIAFSTPKAN