Protein Info for GFF507 in Variovorax sp. SCN45

Annotation: Hydrolase, alpha/beta fold family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 PF12146: Hydrolase_4" amino acids 33 to 269 (237 residues), 34.9 bits, see alignment E=1.5e-12 PF00561: Abhydrolase_1" amino acids 33 to 277 (245 residues), 64.9 bits, see alignment E=1.4e-21 PF12697: Abhydrolase_6" amino acids 36 to 284 (249 residues), 62.9 bits, see alignment E=1.1e-20

Best Hits

KEGG orthology group: None (inferred from 92% identity to vpe:Varpa_3438)

Predicted SEED Role

"D-(-)-3-hydroxybutyrate oligomer hydrolase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (297 amino acids)

>GFF507 Hydrolase, alpha/beta fold family (Variovorax sp. SCN45)
MSDNHNNQIPLSRYAQLAGREIHWLEWGAEDAPVVIAWHGLARTGRDMDELAQHFASRGF
RVICPDTIGRGLSQWSPLPDEEYKLSFYARIANALCDELRLGRVHWVGTSMGGAIGTVCA
SGLFEPRMKARIASLVLNDNAPELAQPAIERIRAYAGQPPAFDTVAELEAFFRTVYKPYG
WLSDAQWRRLTESSTRRLADGRVTPHYDPAMVRQFSAHDNDYLIWPHYDAIEVPVLCLRG
VESDLVLPEVIEEMKRRGPGAAGRLQVIEVPACGHAPALNVPEQLDPVEGFIRAAGR