Protein Info for GFF5066 in Variovorax sp. SCN45

Annotation: Exodeoxyribonuclease III (EC 3.1.11.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 267 TIGR00633: exodeoxyribonuclease III (xth)" amino acids 3 to 263 (261 residues), 234.4 bits, see alignment E=1.5e-73 TIGR00195: exodeoxyribonuclease III" amino acids 3 to 262 (260 residues), 218.1 bits, see alignment E=1.5e-68 PF03372: Exo_endo_phos" amino acids 6 to 256 (251 residues), 89.1 bits, see alignment E=1.8e-29

Best Hits

KEGG orthology group: K01142, exodeoxyribonuclease III [EC: 3.1.11.2] (inferred from 92% identity to vap:Vapar_0277)

Predicted SEED Role

"Exodeoxyribonuclease III (EC 3.1.11.2)" in subsystem DNA repair, bacterial (EC 3.1.11.2)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.2

Use Curated BLAST to search for 3.1.11.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (267 amino acids)

>GFF5066 Exodeoxyribonuclease III (EC 3.1.11.2) (Variovorax sp. SCN45)
VFKLTSLNLNGLRSAATKGVADWVAELAPDCICMQEIRVQASDVEGRFEEMAGLKGHFHF
AEKKGYAGTAVYTKHAPSQVVVGWGDAEFDAEGRYLELRFDTPKRKFSVISCYFPSGSSG
EERQEAKFRFLKGFFPHLVALKKEREFVLCGDINIAHQQIDLKNWRGNQKNSGFLPEERA
WMTRLLDAGTEGAGLVDVYRKLKPDTTDEAYTWWSNRGQAYANNVGWRLDYHLATPAIGA
LARTEQIYKTVKFSDHAPITVEYDFTL