Protein Info for GFF5062 in Sphingobium sp. HT1-2

Annotation: Flavohemoglobin / Nitric oxide dioxygenase (EC 1.14.12.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 PF00042: Globin" amino acids 30 to 130 (101 residues), 45.9 bits, see alignment E=1.1e-15 PF00970: FAD_binding_6" amino acids 163 to 256 (94 residues), 21.7 bits, see alignment E=3.3e-08 PF00175: NAD_binding_1" amino acids 268 to 375 (108 residues), 44.5 bits, see alignment E=3.4e-15

Best Hits

Swiss-Prot: 54% identical to HMP_XYLFT: Flavohemoprotein (hmp) from Xylella fastidiosa (strain Temecula1 / ATCC 700964)

KEGG orthology group: K05916, nitric oxide dioxygenase [EC: 1.14.12.17] (inferred from 84% identity to swi:Swit_3173)

Predicted SEED Role

"Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17)" in subsystem Bacterial hemoglobins or Flavohaemoglobin or Glutaredoxins (EC 1.14.12.17)

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.17

Use Curated BLAST to search for 1.14.12.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (403 amino acids)

>GFF5062 Flavohemoglobin / Nitric oxide dioxygenase (EC 1.14.12.17) (Sphingobium sp. HT1-2)
MTQPLSDQTIALVKATVPALEAHGLDIVNEMYSRMFQNPNIRDLFNQSHHGDAGSQPRAL
TGAILAYASNIENLGALAPAVERIAQKHVGLQILPEHYPHVAEALLGAIKAVLGDAATDE
ILAAWGEAYWFLANILIAREQRVYSEQKDATGGWNGWRDFRVEDVVRESSVINSFVLRPV
DGGAVMRHKPGQYLTFWLEIPGHPPVKRNYSISAAPNGETYRISVKREPLGLASGWLHDE
AKPGTVLRVAAPAGEFFLAAHVERPVILLSGGVGLTPMVAMLETLAEGEAGVPVHYVHGT
HDRDTHAMRDHVRAVAGRGKSITVTDFHQTPLEDEVAGRDYDVAGIITDEWLVANTPVGE
ADYYICGPRPFLRHAVSTLSLAGVPSDRIHYEFFGPADELLAA