Protein Info for GFF506 in Sphingobium sp. HT1-2

Annotation: HAD-superfamily hydrolase, subfamily IA, variant 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 253 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details PF00702: Hydrolase" amino acids 39 to 215 (177 residues), 114.1 bits, see alignment E=2.2e-36 PF13419: HAD_2" amino acids 42 to 221 (180 residues), 116.5 bits, see alignment E=3e-37 PF12710: HAD" amino acids 42 to 169 (128 residues), 38.9 bits, see alignment E=2.5e-13 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 115 to 221 (107 residues), 64.3 bits, see alignment E=1.5e-21 PF13242: Hydrolase_like" amino acids 177 to 222 (46 residues), 23.7 bits, see alignment 7.1e-09

Best Hits

KEGG orthology group: None (inferred from 45% identity to pzu:PHZ_c0876)

Predicted SEED Role

"Beta-phosphoglucomutase (EC 5.4.2.6)" in subsystem Maltose and Maltodextrin Utilization or N-Acetyl-Galactosamine and Galactosamine Utilization or Trehalose Uptake and Utilization (EC 5.4.2.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (253 amino acids)

>GFF506 HAD-superfamily hydrolase, subfamily IA, variant 3 (Sphingobium sp. HT1-2)
MARFAMEILQRTHHMKGMASTASLSDAPPQAAAALSNPIRAVIFDMDGTLLDTEAAHRDA
FARTGAAMGWPMSDEMLLSMVGIHRDENLRMLADRMGPEFPVDQFYADSDALFVAALEAG
IPLRPGAEVILDHLARAGIPMAIATSTMAPYAQQRLEKAGLLPYFQTVVTRNDVDRPKPD
PQPYLLAAERLGVDPAHCVAVEDSHAGVRAGVAAGMATVMIPDLLPPTEELVLAASAVLP
SLHDLRDLLLATE