Protein Info for GFF5054 in Variovorax sp. SCN45

Annotation: Homogentisate 1,2-dioxygenase (EC 1.13.11.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 TIGR01015: homogentisate 1,2-dioxygenase" amino acids 10 to 434 (425 residues), 651.8 bits, see alignment E=2.1e-200 PF20510: HgmA_N" amino acids 11 to 280 (270 residues), 379.8 bits, see alignment E=7.4e-118 PF04209: HgmA_C" amino acids 283 to 434 (152 residues), 263.5 bits, see alignment E=5.2e-83

Best Hits

Swiss-Prot: 64% identical to HGD_MYXXD: Homogentisate 1,2-dioxygenase (hmgA) from Myxococcus xanthus (strain DK 1622)

KEGG orthology group: K00451, homogentisate 1,2-dioxygenase [EC: 1.13.11.5] (inferred from 92% identity to vap:Vapar_0289)

Predicted SEED Role

"Homogentisate 1,2-dioxygenase (EC 1.13.11.5)" in subsystem Homogentisate pathway of aromatic compound degradation (EC 1.13.11.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (436 amino acids)

>GFF5054 Homogentisate 1,2-dioxygenase (EC 1.13.11.5) (Variovorax sp. SCN45)
MTQATPPSERRYQSGFGNEYASEAVPGALPQGRNNPQRGPLDLYTELISGTAFTAPRHEN
RRTWLYRRQPSVVSGRYQPYAQPHWTTGADREIALPPEPLRWHPQPLDGAEGADFIDGMH
TIAANGDAESQVGIGSLMYLAGRSMERRAFVNADGEMLLVPQQGRLVITTELGVLDVKPG
EIAVLPRGMAFKVALPDGLSRGYVCENYGAHFRLPELGPIGSNGLANARDFQAPVAAFET
EEGGYEIVKKFGGRFWQAPMKQSPFNVVAWHGNLSPVKYDTAHFMVIGSISFDHPDPSIF
TVLTSPSDTPGTANCDFVIFPPRWMVMENTFRPPWFHRNLMSEFMGLVLGEYDAKPGGFK
PGGASLHNCMVPHGPDEEAFDKATRADLKPHKLDNTLAFMFESRYRFIPTNFALKSSALD
TDYADCWAGLKDQFKA