Protein Info for GFF504 in Xanthobacter sp. DMC5

Annotation: Histidine N-alpha-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 TIGR03438: dimethylhistidine N-methyltransferase" amino acids 21 to 319 (299 residues), 414.7 bits, see alignment E=1e-128 PF10017: Methyltransf_33" amino acids 21 to 321 (301 residues), 385.9 bits, see alignment E=5.9e-120

Best Hits

Swiss-Prot: 48% identical to EGTD_MYCS2: Histidine N-alpha-methyltransferase (egtD) from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

KEGG orthology group: None (inferred from 68% identity to xau:Xaut_4562)

MetaCyc: 48% identical to L-histidine Nalpha-methyltransferase (Mycolicibacterium smegmatis)
Dimethylhistidine N-methyltransferase. [EC: 2.1.1.44]

Predicted SEED Role

"FIG00636833: hypothetical protein"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.44

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (323 amino acids)

>GFF504 Histidine N-alpha-methyltransferase (Xanthobacter sp. DMC5)
MRASTLVRAEQSPQTPERAAFRADVIEGLSASPKQLAPKYFYDEAGSRLFDLICRLPEYY
PTRTELGVLKAHAGDIAALAGPGASLVEFGSGSSVKVRLLLDALERPAAYVPIDISGLHM
RAAVAALARDYPTVAMTPVEGDFTRPLELPHLGEGGRRIGFFPGSTIGNFTPAEAEAFLT
HARQSLGEGAALVLGFDLPKERAVLEAAYDDHQGVTAAFNLNLLTRINRELGADFDLAAF
HHRALFNEAESRIEMHLESRAAQTVRLDGARFTFAPGETIHTENSYKYAAARLMGMAQAA
GWRQAALWTDPRGWFAVAVLEAV