Protein Info for PS417_25770 in Pseudomonas simiae WCS417

Annotation: ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 664 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 275 to 297 (23 residues), see Phobius details PF00497: SBP_bac_3" amino acids 46 to 263 (218 residues), 50.8 bits, see alignment E=5.3e-17 PF00989: PAS" amino acids 332 to 436 (105 residues), 24.7 bits, see alignment E=7.2e-09 PF13188: PAS_8" amino acids 332 to 382 (51 residues), 23.1 bits, see alignment 1.8e-08 TIGR00229: PAS domain S-box protein" amino acids 332 to 443 (112 residues), 39.4 bits, see alignment E=3.1e-14 PF08448: PAS_4" amino acids 333 to 441 (109 residues), 30.5 bits, see alignment E=1.3e-10 PF13426: PAS_9" amino acids 338 to 438 (101 residues), 33.1 bits, see alignment E=2e-11 PF07730: HisKA_3" amino acids 467 to 533 (67 residues), 53 bits, see alignment E=1.4e-17 PF02518: HATPase_c" amino acids 573 to 663 (91 residues), 48.3 bits, see alignment E=4.4e-16

Best Hits

KEGG orthology group: None (inferred from 96% identity to pfs:PFLU5570)

Predicted SEED Role

"Probable two-component sensor, near polyamine transporter" in subsystem Polyamine Metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UG41 at UniProt or InterPro

Protein Sequence (664 amino acids)

>PS417_25770 ABC transporter substrate-binding protein (Pseudomonas simiae WCS417)
MGFRCLLVIGCLCLALMANAAPAPVAPQAQLNAQQREWLAQHPELRVGLVLQAPFAQYDR
RLQRLSGANVELMQWLAKALNIELTWRNFPNQEQLEAALSDGEVDIAPGLQQTPAGLRLW
LFSDPYMRVPQHIVGIRDGGGAVELEKLDEQSRVAVRMPSAVADYLRSTYPGLNLQGVPM
ERQALQLLVSQQARYAVVDEAQLSRLSGEAEFAGLAVVGDIGLPQLLRVATRREWPELAS
IMESALRAIPARDLDQLHSRWLQPKYPRLTESPGLWQNLSLLLGLLLLASLAAVFWLRRQ
QRVLEHNLLAAREESAARAAGAEALRLTQFSIDQSTVGILWVNWDSHVRYANRAAESMLG
YGPGALIERPLVALDPSLDMDRWLNLWKRARASEDGPQNFATDCRRADGSILPTNVSLSF
LRFAEAEYLVVYLNDVTELRRTLAALLQSEAQLRELSAHLETVREEEKARIAREVHDELG
QMLTVLKLETSMCELAYAQLDPGLHERLNSMKRLIAQLFQLVRDVATALRPPILDAGIAS
AIEWQARRFEARTQIPCLVQVPDNLPTLSDAKAIGLFRILQEALTNVMRHAQAHTVELTL
ALEGTDLRLTISDDGVGFVQAQGRPVSFGLVGMRERVLIMGGQLSLDSELGEGTTLSVTV
PLDA