Protein Info for GFF503 in Variovorax sp. SCN45

Annotation: Ferrochelatase, protoheme ferro-lyase (EC 4.99.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 70% identical to HEMH_POLSJ: Ferrochelatase (hemH) from Polaromonas sp. (strain JS666 / ATCC BAA-500)

KEGG orthology group: K01772, ferrochelatase [EC: 4.99.1.1] (inferred from 95% identity to vpe:Varpa_3435)

MetaCyc: 44% identical to ferrochelatase (Escherichia coli K-12 substr. MG1655)
PROTOHEMEFERROCHELAT-RXN [EC: 4.98.1.1]

Predicted SEED Role

"Ferrochelatase, protoheme ferro-lyase (EC 4.99.1.1)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 4.99.1.1)

MetaCyc Pathways

superpathway of heme b biosynthesis from glutamate (9/10 steps found)
superpathway of heme b biosynthesis from glycine (6/8 steps found)
heme b biosynthesis I (aerobic) (3/4 steps found)
heme b biosynthesis II (oxygen-independent) (3/4 steps found)
heme b biosynthesis V (aerobic) (3/4 steps found)
superpathway of heme b biosynthesis from uroporphyrinogen-III (4/6 steps found)

KEGG Metabolic Maps

Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.98.1.1 or 4.99.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (343 amino acids)

>GFF503 Ferrochelatase, protoheme ferro-lyase (EC 4.99.1.1) (Variovorax sp. SCN45)
MPPHPETAQPGTPRTAVLWCNLGSPDAPTAAAVRPYLADFLGDPRVVEIPKAIWALILHG
IILRTRPAKSAAKYASIWTPEGSPLKVFTQKQATLLAGWLGERGHRVTVRDAMRYANPSI
ASRLDALQAEGATRVLVLQAYPQYSATTTASVIDAVNDWSRKQRRLPEFRFVNDFHDDPA
YIEALAQGIEKHWKTEGRGEVLLMSFHGIPARNIALGDPYQSQCLETARLLAARLGLSAA
QHRVTFQSRFGRAKWLEPYTEPTLRELGASGIQRVDVVCPGFPADCLETLEEIAMEGREA
FLHAGGKAFSYIPCLNDSPAWITALAGIAERNLAGWPTQAFTR