Protein Info for GFF5007 in Sphingobium sp. HT1-2

Annotation: Thymidine phosphorylase (EC 2.4.2.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 512 TIGR02645: putative thymidine phosphorylase" amino acids 34 to 506 (473 residues), 557.5 bits, see alignment E=1.1e-171 PF02885: Glycos_trans_3N" amino acids 114 to 170 (57 residues), 33.6 bits, see alignment 4.1e-12 PF00591: Glycos_transf_3" amino acids 180 to 407 (228 residues), 44.2 bits, see alignment E=2.8e-15 PF07831: PYNP_C" amino acids 443 to 498 (56 residues), 31.7 bits, see alignment 1.5e-11

Best Hits

Swiss-Prot: 66% identical to TYPH_SPHAL: Putative thymidine phosphorylase (Sala_2473) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K00758, thymidine phosphorylase [EC: 2.4.2.4] (inferred from 99% identity to sjp:SJA_C1-04780)

Predicted SEED Role

"Thymidine phosphorylase (EC 2.4.2.4)" in subsystem Deoxyribose and Deoxynucleoside Catabolism (EC 2.4.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.4

Use Curated BLAST to search for 2.4.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (512 amino acids)

>GFF5007 Thymidine phosphorylase (EC 2.4.2.4) (Sphingobium sp. HT1-2)
VKPGIIEDAEVEPVATTLRAHRLGLSAAGGDLIAVMRHDCPVCRSEGLASRAQVALRAGG
REIVVSLLHSSAETPAPGEIGLSESAWQRLGVSDGDPVEIAHAQPLASLAEVRRRIYGNR
LSEGAFSAIMTDIAERRYSDVHLSAFITACSAVPLDTDETISLTRAMVDVGERLQWSGAV
IVDKHSVGGLPGNRTTPIIVSIMAAEGLIMPKTSSRAITSPAGTADTMEVLAPVDLDVSA
IRKVVEREGGCIAWGGAVNLSPADDVIIGVERVLDIDAVGQMVASVLSKKIAAGATHLVI
DIPVGPTAKVRGTDAADTLERALSSVAQAFGLRTRVMRGPGAEPIGRGIGPALEAQDILA
VLQGQPGAEDLAHRACELAGGLLELADAAPAGEGYARARACLESGGAWAKFQRICEAQGG
MRAPPVARFQQDMIAPYSGRLVSIDNRKLATVAKLAGAPVAKAAGVALQCRLNQMVDAGA
PLCTIHAESPGELDYAAAYAVSDGPIFGIEAL