Protein Info for GFF5004 in Variovorax sp. SCN45

Annotation: N-acyl-L-amino acid amidohydrolase (EC 3.5.1.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 TIGR01891: amidohydrolase" amino acids 16 to 361 (346 residues), 362.8 bits, see alignment E=1e-112 PF01546: Peptidase_M20" amino acids 73 to 355 (283 residues), 153.3 bits, see alignment E=8.9e-49 PF07687: M20_dimer" amino acids 188 to 281 (94 residues), 45.1 bits, see alignment E=8.6e-16

Best Hits

Swiss-Prot: 41% identical to CBPX1_SACS2: Thermostable carboxypeptidase 1 (cpsA1) from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 95% identity to vap:Vapar_0333)

Predicted SEED Role

"Catalyzes the cleavage of p-aminobenzoyl-glutamate to p-aminobenzoate and glutamate, subunit A" in subsystem p-Aminobenzoyl-Glutamate Utilization

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32

Use Curated BLAST to search for 3.5.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (400 amino acids)

>GFF5004 N-acyl-L-amino acid amidohydrolase (EC 3.5.1.14) (Variovorax sp. SCN45)
MKLIDSLVTQAAGIAAVRRDLHAHPELCFEEVRTADVVAGKLTEWGIPIHRGLGTTGVVG
IVKNGTSSRAVGLRADMDALPVTELNTFAHASKHHGKMHACGHDGHTAMLLAAAQHLAKN
RNFDGTVYLIFQPAEEGGGGAREMIKEGLFEQFPMDAVFGMHNWPGMKAGQFAVSPGPVM
ASGNKFFVNVIGKGGHAALPQTGIDPVPIACEIVQAFQTILTRKMKPTDSAVISVTTIHA
GETNNVIPDNCELSGTVRTFSLEVLDLIEAKMKQIADHICAAHDATCDFRFERYYPPTVN
TEAEADFARRVMGGIVGSENVLKQEAAMTSEDFAFMLQAKPGAYAFIGNGDGAHRDVHHG
EGPCTLHNASYDFNDDLIPLGATCWVQIAEQFLKPAAAAE