Protein Info for GFF4968 in Variovorax sp. SCN45

Annotation: Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 574 PF00890: FAD_binding_2" amino acids 13 to 551 (539 residues), 284.3 bits, see alignment E=7.3e-88 PF12831: FAD_oxidored" amino acids 13 to 65 (53 residues), 36.7 bits, see alignment 1.2e-12 PF01266: DAO" amino acids 13 to 91 (79 residues), 36.4 bits, see alignment E=1.6e-12 PF13450: NAD_binding_8" amino acids 16 to 50 (35 residues), 29.5 bits, see alignment (E = 2.6e-10)

Best Hits

KEGG orthology group: None (inferred from 66% identity to axy:AXYL_00521)

Predicted SEED Role

"Fumarate reductase flavoprotein subunit (EC 1.3.99.1)" in subsystem Succinate dehydrogenase (EC 1.3.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.1

Use Curated BLAST to search for 1.3.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (574 amino acids)

>GFF4968 Succinate dehydrogenase/fumarate reductase, flavoprotein subunit (Variovorax sp. SCN45)
MAQQQQQEKTEYDVVVIGSGAGGLSAALTARLEGLDVLVVEKTDRIGGSTAISGGAVWVP
LNDQAEAAGHPDTRGKAWTYLQNTVGAAGDEELRHAFLDAGPLALRYLRERTDVQLAART
YSPDYYPDREGAAMGGRSLDPIEFDGRLLGPHFKTLRDPLPEFCVLGGMMVNMTDVKHLL
GVWRSFASWKHGVKLVLRYFGDRLGGHHRGTRLLLGNALAARLFHSVLKTGIPFWLNTPA
LGLEKDATGAVTGVRLRRDGKELTVRARRGVVIATGGFPWNAAMRGQHYPAPTGPYSMSP
QDNAGEGIAMAQQAGGVLGTGHTGPALWAPVSLLTRPDGSVLRYPHLVWDRAKPGLIAVD
ARGERFVNESTSYHEFVRGMYRANAKAASIPALLVCDSAFMEKWGLGLALPGGRPREHLV
RAGYLYRADTLEALAQQAGVDPAGLARSVAKFNPLAAQGQDPVFGKGGDAYNRYLGDPDH
QPNACLAPVKQAPFYAVKVYPGDIGTALGIRADGNARALDAQGEPIAGLYVAGNDMHSVM
GGEYPAPGITLGPALTFGWVAGMHLARGAAAEHH