Protein Info for GFF4957 in Sphingobium sp. HT1-2

Annotation: Adenosylhomocysteinase (EC 3.3.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05221: AdoHcyase
  • amino acids 11 to 471 (461 residues), 484.8 bits, E=1.9e-149
• TIGR00936: adenosylhomocysteinase
  • amino acids 12 to 464 (453 residues), 581.8 bits, E=3.7e-179
• PF00670: AdoHcyase_NAD
  • amino acids 233 to 392 (160 residues), 256.8 bits, E=1.9e-80
• PF02826: 2-Hacid_dh_C
  • amino acids 252 to 342 (91 residues), 28.3 bits, E=2.2e-10
• PF07991: KARI_N
  • amino acids 253 to 319 (67 residues), 25 bits, E=2.6e-09

Best Hits

Swiss-Prot: 84% identical to SAHH_SPHAL: Adenosylhomocysteinase (ahcY) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K01251, adenosylhomocysteinase [EC: 3.3.1.1] (inferred from 88% identity to sch:Sphch_0733)

Predicted SEED Role

"Adenosylhomocysteinase (EC 3.3.1.1)" in subsystem Methionine Biosynthesis or Methionine Degradation (EC 3.3.1.1)

MetaCyc Pathways

• S-adenosyl-L-methionine salvage II (3/3 steps found)
• L-methionine degradation I (to L-homocysteine) (2/3 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (2/5 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Selenoamino acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.3.1.1

Protein Sequence (472 amino acids)

>GFF4957 Adenosylhomocysteinase (EC 3.3.1.1) (Sphingobium sp. HT1-2)
VATVPADKAQDYVIADIGLAAFGRKEIEIAETEMPGLMALRAEFGASQPLKGARITGSLH
MTIQTAVLIETLTALGAQVRWATCNIYSTQDHAAAAIAASGVPVFAVKGESLQEYWDYVE
RIFDWHNDDSGVCNLILDDGGDATMFALWGARVEAGEELFTPSNEEEEIFCAVLKRVLAE
RPGFLTKTVQSIKGVSEETTTGVHRLYELSKKGKLPFPAINVNDSVTKSKFDNLYGCKES
LVDAIRRGTDVMLAGKVACVAGFGDVGKGSAASLRNGGARVLVTEIDPICALQAAMEGYE
VVTMEEAAPRADIFVTATGNEGVLTVDHMRAMKNMAIVSNIGHFDSEIEIAGLANMKWTE
IKPQVDEVEFPDGKKIIVLSKGRLVNLGNATGHPSFVMSASFTNQTLAQIELWTKSETYG
NDVYVLPKHLDEKVAALHLEKLGVKLTQLSQRQADYIGVPVEGPFKPDHYRY