Protein Info for GFF4947 in Sphingobium sp. HT1-2

Annotation: Glutamate N-acetyltransferase (EC 2.3.1.35) @ N-acetylglutamate synthase (EC 2.3.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00120: glutamate N-acetyltransferase/amino-acid acetyltransferase
  • amino acids 22 to 412 (391 residues), 349.6 bits, E=1.1e-108
• PF01960: ArgJ
  • amino acids 30 to 412 (383 residues), 449 bits, E=5.6e-139

Best Hits

Swiss-Prot: 69% identical to ARGJ_ZYMMO: Arginine biosynthesis bifunctional protein ArgJ (argJ) from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)

KEGG orthology group: K00620, glutamate N-acetyltransferase / amino-acid N-acetyltransferase [EC: 2.3.1.1 2.3.1.35] (inferred from 91% identity to sch:Sphch_0724)

Predicted SEED Role

"Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1)" in subsystem Arginine Biosynthesis extended (EC 2.3.1.1, EC 2.3.1.35)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• L-ornithine biosynthesis I (4/5 steps found)
• superpathway of arginine and polyamine biosynthesis (12/17 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.1 or 2.3.1.35

Protein Sequence (412 amino acids)

>GFF4947 Glutamate N-acetyltransferase (EC 2.3.1.35) @ N-acetylglutamate synthase (EC 2.3.1.1) (Sphingobium sp. HT1-2)
MPRPMTDRSPLAPAAFPALPAIAGVTLRVAKARYKNWDRCDLTYVELDAGTAVAGVTTRS
KCPSPEVEWCRDAIPMGQARAVVVNAGNANAFTGHRGRAAVEAIAAKVANHLSCQPSDIF
VSSTGVIGVPLPIDKAEAGLEAAFVAAPCGWEDAANTIGTTDTYAKGAHVSAMIGDTRVD
LVGIIKGSGMIAPDMATMLGYIFTDAAIDPALLQQMLSAANKRTFSCITVDSDTSTSDTV
LAFATGKAGNAPLASMEDAGADAFAAALSDLCRQLAHLVVRDGEGASKFIEITVEGAESD
ASAHRIALSIANSPLVKTAIAGEDANWGRVVMAVGKAGEPAERDKLAIRFGATQVAAGGL
ALDGYDEAPVAAHLKGQDIVIGVDIGLGEGRATMWTCDLTHGYISINADYRS