Protein Info for GFF4945 in Sphingobium sp. HT1-2

Annotation: Protein translocase subunit SecA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 910 PF07517: SecA_DEAD" amino acids 7 to 388 (382 residues), 437.7 bits, see alignment E=4.8e-135 TIGR00963: preprotein translocase, SecA subunit" amino acids 28 to 811 (784 residues), 1105.4 bits, see alignment E=0 PF01043: SecA_PP_bind" amino acids 232 to 345 (114 residues), 145.3 bits, see alignment E=2.6e-46 PF21090: P-loop_SecA" amino acids 404 to 608 (205 residues), 301.1 bits, see alignment E=9.5e-94 PF07516: SecA_SW" amino acids 614 to 823 (210 residues), 223.4 bits, see alignment E=7.7e-70 PF02810: SEC-C" amino acids 890 to 907 (18 residues), 38.7 bits, see alignment (E = 1.8e-13)

Best Hits

Swiss-Prot: 77% identical to SECA_SPHAL: Protein translocase subunit SecA (secA) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K03070, preprotein translocase subunit SecA (inferred from 92% identity to sch:Sphch_0723)

Predicted SEED Role

"Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (910 amino acids)

>GFF4945 Protein translocase subunit SecA (Sphingobium sp. HT1-2)
MFGALAKSIFGSSNERYVKSLGKVIDQIAAFEPTIQAMSDDELVSQTARFRERLANGETL
DGLLPEAFATVREASVRVLGMRHFDVQMIGGIVLHRGEIAEMRTGEGKTLVATLATYLNA
LEGKGVHVVTVNDYLASRDCEWMGQVYRFLGLTTGVIVPNLSEDQRREAYNADITYATNN
ELGFDYLRDNMKFDRGAMVQRPFNHAIVDEVDSILIDEARTPLIISGPTDDKSELYVAVN
AIVKQLVEEDYEKDEKQRTITLTEDGTEKIERLLEAAGLLQGNNLYDFENTQVVHHVNQA
LRAVVMFRRDIDYIVKDGKVVIIDEFTGRMMDGRRWSDGLHQAVEAKEGVQIEPENQTLA
SITFQNYFRMYPKISGMTGTASTEATEFYEIYKMNVVTIPTNRPVQRVDEEDTFYKNLED
KFRGIARTIKEHAEKGQPVLVGTVSIEKSEMLSEFLNQEGVKHAVLNARFHESEAHIVAQ
AGRKGAVTIATNMAGRGTDIKLGGNLEMRVEDELRDMPEGPEREAAIARIEVEIEAEKQE
VLAAGGLFVLATERHESRRIDNQLRGRSGRQGDPGLSRFYLSLDDDLMRIFGPDTMFAKM
IRSNLEDGEALPPSKWLSKAIETAQRKVEARNYDIRKQVVEYDDVMNDQRKVIYEQRSDI
MDAETVDDVVTDMRHETVNDLVGASCPPGTYPEQWDMERLKLRTAEVLGLEPDFDAWLAE
DAVDPEMIEERLAGLADAAIAEKTQEVEAADWHMIEKSIMLQSLDHHWKEHLSTLDALRQ
VVHLRAYAQKTPINEYKQEAFALFERMLENIREDVTSSIARVQFRMEAPQPQFDLPVLPD
FITTHIDPFSGEDNSADIDGSQLAGITTTIPRAPVDNVAPGEFANLDISRNALCPCGSGQ
KYKHCHGALS