Protein Info for GFF494 in Sphingobium sp. HT1-2

Annotation: GAF domain/GGDEF domain/EAL domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 505 PF01590: GAF" amino acids 27 to 156 (130 residues), 52.1 bits, see alignment E=2.5e-17 TIGR00229: PAS domain S-box protein" amino acids 178 to 299 (122 residues), 42.2 bits, see alignment E=4.2e-15 PF08448: PAS_4" amino acids 189 to 296 (108 residues), 37.4 bits, see alignment E=6.6e-13 PF08447: PAS_3" amino acids 205 to 288 (84 residues), 75.1 bits, see alignment E=1.1e-24 PF07568: HisKA_2" amino acids 308 to 358 (51 residues), 22.4 bits, see alignment 2.7e-08 PF07536: HWE_HK" amino acids 308 to 393 (86 residues), 49.3 bits, see alignment E=1.7e-16

Best Hits

KEGG orthology group: None (inferred from 74% identity to sch:Sphch_1571)

Predicted SEED Role

"putative signal transduction histidine kinase with PAS/PAC domains"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (505 amino acids)

>GFF494 GAF domain/GGDEF domain/EAL domain protein (Sphingobium sp. HT1-2)
MADGRGLATDRAALLALYDLDAGGFRTLDDITSFAAQLCGAPIALVSIVEDVRQRFLARV
GLDAEETPRDVSFCALAMLGDNIFVVPDATQDARFIDNALVTGAPHIRFYAGAPLVGGDG
EPLGALCVIDTVPRDDLTPLQRQGLSLLARQVMVELEGRRRDRDVIAQQRLDAAAVADSD
RMFRTLADTMPQMVWSTLPDGYHDYYNARWYEYTGVPAGSTDGDAWNGMFHADDQPKAWE
KWRHSLASGDPYEIEYRLRHRDGLYRWTLGRALPIRDAQGQIIRWIGTCTEIHEQKLMLE
EREMIAHELSHRIKNIFSVIAGLIGLSARERPGMQEGADDLRDRILALGRAHDFVRTHGG
GVSMADEQASLHGVLEEIFAPYGGRERLQLSGDNPPIDDRSATPLALLFHELATNAAKYG
ALSTPDGFIALHVVTQDEDILLDWREEGGPSVAPIASEGFGSRLIALSVERQLGGQLERQ
WCPEGLRLSLRIPRRSMHRAAAGRA