Protein Info for GFF4933 in Sphingobium sp. HT1-2

Annotation: Butyryl-CoA dehydrogenase (EC 1.3.99.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 736 PF02771: Acyl-CoA_dh_N" amino acids 6 to 103 (98 residues), 58.4 bits, see alignment E=1.9e-19 amino acids 358 to 469 (112 residues), 54 bits, see alignment E=4.2e-18 PF00441: Acyl-CoA_dh_1" amino acids 201 to 335 (135 residues), 73.4 bits, see alignment E=4.5e-24 amino acids 610 to 727 (118 residues), 65.2 bits, see alignment E=1.6e-21 PF02770: Acyl-CoA_dh_M" amino acids 474 to 552 (79 residues), 61 bits, see alignment E=2e-20

Best Hits

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2

Use Curated BLAST to search for 1.3.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (736 amino acids)

>GFF4933 Butyryl-CoA dehydrogenase (EC 1.3.99.2) (Sphingobium sp. HT1-2)
MNFDLTDDQEMMRDMFARFLDEHSSMARVRAAAPTGFDPALWQGLAELGALSIRVPEEAG
GLGLGLFDATILMEEAGRTLASGPLAEALVAARLLAQLGGEATADLLGRVLAGDAVASFA
FDDIADTPVQWIGGGLVAEAVLARSGEDIILVTVPAEARKAEDNLASTPIAQIDLSAHPC
TVLASGAEALATFAAGIEEWKLLIATGLAGIGREALKLAAAYACERKQFDQYIGQFQAIS
HPLADILCEIDGGKFLVWKAIRDLADGAPEAGGAISIAAWWNANSAAKAVAQSLHTFGGY
GLTTEYDIFLYNLRAKAWPLIGGDPQRWLDEAGRRLYADEAAALPDAGPMPVDFDLGEAA
QAIKDEINAFFAANVTPEMRATFHYSWEGYNPELNRKLAAQNLLYLGLPKDVGGRGLSAY
EKTAAMDAFEEQGYNNPAANVTQMVSLIIHRFGTEELKQAVLPEIMRGEVICSLGYSEPG
SGSDVFAAQCRATQEGDGSWRIDGTKMFTSGANLSTYVLMLCRTNPDVAKHKGLTMFIVP
LKADGVTVQPVHTFQDERTNITFYDGVRIPDSWRLGEIDGGVRTMSASLELEHGGGFAKY
QRKMLQAGEALCRDVIHRGRPLIEDVGAQARLARTAANLWASELITYRAQWVMSEKKPNH
AYGPMAKMFSSEKFLTDARDLLDLTAPASLSKRKGPAEEINMFYRHAQGSTIYGGTSEVH
RSMIAEKGLGLPRTRA