Protein Info for PS417_25260 in Pseudomonas simiae WCS417

Annotation: riboflavin synthase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 220 TIGR00187: riboflavin synthase, alpha subunit" amino acids 1 to 197 (197 residues), 223.2 bits, see alignment E=9.6e-71 PF00677: Lum_binding" amino acids 3 to 87 (85 residues), 71.8 bits, see alignment E=1.9e-24 amino acids 100 to 184 (85 residues), 94.6 bits, see alignment E=1.5e-31

Best Hits

Swiss-Prot: 59% identical to RISA_PHOPO: Riboflavin synthase (ribE) from Photobacterium phosphoreum

KEGG orthology group: K00793, riboflavin synthase [EC: 2.5.1.9] (inferred from 99% identity to pfs:PFLU5472)

MetaCyc: 46% identical to riboflavin synthase monomer (Bacillus subtilis)
Riboflavin synthase. [EC: 2.5.1.9]

Predicted SEED Role

"Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9)" (EC 2.5.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UCV4 at UniProt or InterPro

Protein Sequence (220 amino acids)

>PS417_25260 riboflavin synthase subunit alpha (Pseudomonas simiae WCS417)
MFTGIIESIGSIRAMTPKGGDVRLLVETGKLDLGDVKLGDSIAVSGVCLTVIELPGNGFA
ADVSRETLDCTAMNDLKAGSPVNLEKALTPTTRLGGHLVSGHVDGVGEVVARSENARAVE
FRIRAPKELAKYIAHKGSITVDGTSLTVNAVNGAEFELTIIPHTLSETIMASYQPGRRVN
LEVDLLARYLERLLLGDKAAEPATGNITESFLAANGYLKS