Protein Info for GFF4925 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 770 PF13328: HD_4" amino acids 43 to 228 (186 residues), 140.2 bits, see alignment E=1.3e-44 TIGR00691: RelA/SpoT family protein" amino acids 56 to 768 (713 residues), 537.4 bits, see alignment E=2.7e-165 PF04607: RelA_SpoT" amino acids 287 to 396 (110 residues), 136.7 bits, see alignment E=1e-43 PF02824: TGS" amino acids 439 to 496 (58 residues), 74.9 bits, see alignment 1e-24 PF13291: ACT_4" amino acids 694 to 769 (76 residues), 58.9 bits, see alignment E=1.7e-19

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 75% identity to pol:Bpro_2703)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (770 amino acids)

>GFF4925 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSLTPSDSANAAVLAERAPVVPSAIVAATSDSLPKQANALARARAFAEPLLGSEQLDTGE
NTLAHADAVAGVLHDIGGSEAMQAAAYLVYACQHLNRPQEVIAKAFGDNFAALALETTKL
VQLQRQARIKSAEVLAKKEEERLAAQEQALPVTTPGKTPVSELAASQTENVRKMLLAFSR
DLRVVMLRLASRLQTLRYFAASKGEPGDALASESLHVFAPLANRLGIWQIKWEMEDLAFR
FLEPQTYKQVARLLDEKRTEREAHVEQVRQQLESELLRQGVAASVQGRPKHIYSIVKKMR
GKSLDFDQVFDIRALRVIVPDRDDCYTALAHVHAHYLPVADEFDDYIAKPKPNGYQSLHT
VVRDEQGRAFEIQIRTQAMHDHAEHGVAAHWAYKEAGAKGYAGVSASSEYDAKIAVLRQL
LAWERDLSGTAQGLFDDRIYVLTPDAAIVELPQGATAVDFAYSVHTDLGHRCRGARVDGA
MVTLNTPLQNGQTVEITAAKEGGPSRDWLNAELGYLVSHRAKSKVRAWFNAQAMEETVAR
GREAVEKLLQREGKTALKFDDLAVSMGLASAQELFEQVGKDELSLRAIETHLRPPEPEAP
SEIPVWLKKPRKSSSQPSGGVLVVGVDSLMTQLAKCCKPAPPDEIGGFVTRGKGVSIHRA
DCSDFAHLQRKNPDRVIEVQWSGQGAPDRDGRAAVYPVDVAIEAQDRQGLLRDISDVFAR
EKMNVIGVQTQSVRDVAWMTFTIEVSDARQVSRAMSVVGDVPGVRVVRRK