Protein Info for PS417_25240 in Pseudomonas simiae WCS417

Annotation: thiamine monophosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01379: thiamine-phosphate kinase
  • amino acids 2 to 317 (316 residues), 307.2 bits, E=5.7e-96
• PF00586: AIRS
  • amino acids 28 to 138 (111 residues), 103.5 bits, E=9.2e-34
• PF02769: AIRS_C
  • amino acids 150 to 299 (150 residues), 33.2 bits, E=5.7e-12

Best Hits

KEGG orthology group: K00946, thiamine-monophosphate kinase [EC: 2.7.4.16] (inferred from 91% identity to pfs:PFLU5468)

Predicted SEED Role

"Thiamine-monophosphate kinase (EC 2.7.4.16)" in subsystem Thiamin biosynthesis (EC 2.7.4.16)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• thiamine diphosphate biosynthesis I (E. coli) (2/2 steps found)
• thiamine diphosphate biosynthesis II (Bacillus) (2/2 steps found)
• thiamine diphosphate salvage II (4/5 steps found)
• thiamine diphosphate salvage V (2/3 steps found)
• thiamine diphosphate salvage I (1/2 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.16

Sequence Analysis Tools

See A0A1N7U9Q6 at UniProt or InterPro

Protein Sequence (321 amino acids)

>PS417_25240 thiamine monophosphate kinase (Pseudomonas simiae WCS417)
MGEFELIRNYFAAAPCAQGGEGIALGIGDDCALLALPAGEQLAVSTDTLVAGVHFADPCD
PFLLGQRSLAVAVSDLAAMGAHPLAFTLALTTPTVNADWLQRYAEGLNLMAQRCGVGLVG
GDTTRGPLSLTLTVFGRVPAGQALTRSGARPGDLLCVGGELGNAAGALSLVLGQRNADAA
VADPLLAHYWSPQPQLALGLALRGKATAAMDISDGLLADCGHIATASAVSLLIERQRLPL
SQALLAFVGEDAARVAALSGGDDYVLVFTLPSDELASLLADGWPVHVIGRVEAGQGVTLL
DGNGHDITPAIHGYQHFQQTP