Protein Info for GFF4916 in Sphingobium sp. HT1-2

Annotation: Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 32 to 219 (188 residues), 146.8 bits, E=1.8e-46
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 34 to 440 (407 residues), 670.5 bits, E=1.4e-205
• TIGR00231: small GTP-binding protein domain
  • amino acids 40 to 184 (145 residues), 43.3 bits, E=4.9e-15
• PF03144: GTP_EFTU_D2
  • amino acids 271 to 330 (60 residues), 32.7 bits, 2.6e-11
• PF22594: GTP-eEF1A_C
  • amino acids 342 to 440 (99 residues), 105.4 bits, E=5.5e-34
• TIGR00455: adenylyl-sulfate kinase
  • amino acids 453 to 634 (182 residues), 255.2 bits, E=4.7e-80
• PF01583: APS_kinase
  • amino acids 472 to 619 (148 residues), 229.6 bits, E=5.2e-72
• PF13671: AAA_33
  • amino acids 473 to 585 (113 residues), 27.4 bits, E=1.2e-09

Best Hits

Swiss-Prot: 62% identical to NODQ_RHIS3: Bifunctional enzyme NodQ (nodQ) from Rhizobium sp. (strain N33)

KEGG orthology group: K00955, bifunctional enzyme CysN/CysC [EC: 2.7.1.25 2.7.7.4] (inferred from 83% identity to sch:Sphch_3380)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• sulfite oxidation III (1/3 steps found)
• selenate reduction (2/5 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (1/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (1/6 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (1/6 steps found)
• superpathway of seleno-compound metabolism (8/19 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (2/12 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Protein Sequence (640 amino acids)

>GFF4916 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) (Sphingobium sp. HT1-2)
MSDVITQDPVYQTDALIAQDIDAYLDVHQHKTMLRFITCGSVDDGKSTLIGRLLYDSKMI
FEDQLEALQADSKRVGTQGGEIDFALLVDGLAAEREQGITIDVAYRFFATEKRKFIVADT
PGHEQYTRNMVTGASTADLAVILIDARKGILTQTRRHSYLAHLIGIRNIVLAVNKMDLVG
YDQAVFDKIVADYAAFAQSIGISAFTPIPISGFKGDNITARSENTPWYQGPTLMAHLESV
EVDATTAAAKPFRMPVQWVNRPNLDFRGFAGLIASGSVKPGDAVRVLPSGKTSTISRIVT
LDGDLDEAIAGQSVTLCFADEIDCSRGDVIAVADNPPEVSSQFEATIVWMDDEAMLPGRP
YWLKIGTQSVSATVQAPKYVVNVNTMEHLAAKTLDLNAIGVAELATDKPITFEPYADNRT
LGGFILIDKISNRTVAAGMLHFSLRRAQNVHWQATDIGREAHAALKNQLPRILWFTGLSG
SGKSTIANEVEKRLALMNRHTFLLDGDNIRHGLNKDLGFTEADRIENIRRVGEVAKLMAD
AGLIVLTAFISPFRAEREMVREMLPDGEFIEIFVDTPLEVAEARDVKGLYKKARSGSLKN
FTGIDSPYEAPTSPEIRVNTVEMTPEEAAEHIIRQIMPLK