Protein Info for GFF4914 in Variovorax sp. SCN45

Annotation: Luciferase-like monooxygenase YhbW

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03558: luciferase family oxidoreductase, group 1
  • amino acids 4 to 323 (320 residues), 460 bits, E=2.2e-142
• PF00296: Bac_luciferase
  • amino acids 8 to 300 (293 residues), 147.7 bits, E=2.6e-47

Best Hits

Swiss-Prot: 59% identical to YHBW_ECOLI: Luciferase-like monooxygenase (yhbW) from Escherichia coli (strain K12)

KEGG orthology group: K00494, alkanal monooxygenase (FMN-linked) [EC: 1.14.14.3] (inferred from 96% identity to vpe:Varpa_0412)

Predicted SEED Role

"Bacterial luciferase family protein"

MetaCyc Pathways

• bacterial bioluminescence (5/8 steps found)

Isozymes

Compare fitness of predicted isozymes for: 1.14.14.3

Use Curated BLAST to search for 1.14.14.3

Protein Sequence (332 amino acids)

>GFF4914 Luciferase-like monooxygenase YhbW (Variovorax sp. SCN45)
MIPLSILDLSPITEGSDAAQSFRNSLSLAQHGEKLGYTRYWLAEHHGMPGIASAATAVLL
SHVGAGTSTIRIGAGGVMLPNHSPLVIAEQFGTLESLYPGRVDLGLGRAPGSDQRTARAL
RRNLESDSDQFPQDVIELMDFMSKNPQQAVRAVPGAGLEVPVWILGSSTFGAQLAAHLGL
PYAFASHFAPQQIMQAIQIYRETFKPSAQLQKPYVMLGFNVFAADTDEEAEFRATSWQQA
FVNLRSGRPGRLPPPVENYRQKVGPAENALLDSVLSCSAVGSVETVKKGVEAFVARTGAD
ELMITSQVFDHAARLRSYELLAAAFGQTAKQQ