Protein Info for GFF4906 in Xanthobacter sp. DMC5

Annotation: Silver exporting P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 798 transmembrane" amino acids 140 to 160 (21 residues), see Phobius details amino acids 173 to 192 (20 residues), see Phobius details amino acids 203 to 227 (25 residues), see Phobius details amino acids 241 to 261 (21 residues), see Phobius details amino acids 397 to 418 (22 residues), see Phobius details amino acids 424 to 447 (24 residues), see Phobius details amino acids 740 to 759 (20 residues), see Phobius details amino acids 765 to 785 (21 residues), see Phobius details PF04945: YHS" amino acids 32 to 77 (46 residues), 55.2 bits, see alignment 1.6e-18 PF19335: HMBD" amino acids 95 to 121 (27 residues), 46.7 bits, see alignment (E = 7.2e-16) TIGR01511: copper-translocating P-type ATPase" amino acids 186 to 790 (605 residues), 602.4 bits, see alignment E=1.9e-184 TIGR01525: heavy metal translocating P-type ATPase" amino acids 205 to 789 (585 residues), 633.6 bits, see alignment E=7.4e-194 TIGR01494: HAD ATPase, P-type, family IC" amino acids 250 to 758 (509 residues), 314.1 bits, see alignment E=2.6e-97 PF00122: E1-E2_ATPase" amino acids 279 to 459 (181 residues), 193.9 bits, see alignment E=5.5e-61 PF00702: Hydrolase" amino acids 477 to 694 (218 residues), 125.3 bits, see alignment E=1.3e-39 PF12710: HAD" amino acids 616 to 690 (75 residues), 27.3 bits, see alignment E=1.4e-09

Best Hits

Swiss-Prot: 68% identical to SILP_SALTM: Silver exporting P-type ATPase (silP) from Salmonella typhimurium

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 90% identity to xau:Xaut_4834)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (798 amino acids)

>GFF4906 Silver exporting P-type ATPase (Xanthobacter sp. DMC5)
MTERDTHAGHHHHHDHAVHPPEGTRTAPAETVKDPVCGMAVNPATAGFQSEHRGKSYFFC
SAKCHDKFDANPSAYAAGAAETPPATKGQPTGVIYTCPMHPQIRQVGPGNCPICGMTLEP
EVATAESGPSAELVDMTRRFWIGLALALPVLALEMGGHLTNLHMLLGAQASNWTQLVLAT
PVVLWAGWPFFVRAWQSLVTRHLNMFTLIAMGTGVAWLYSVIATAAPQIFPVTFRAADGS
VAIYFEAAAVITVLVLLGQVLELRAREQTGGAIRALLDLAPKSARRVRDDGSDEDVALEA
VAVGDRLRVRPGEKVPVDGELVEGRSSVDESMITGESMPVTKEVGAKVIGGTLNQSGGFV
MRAGKVGRDTMLAQIVHMVAEAQRSRAPIQRLADEVSGWFVPVVIAIALLAFAAWGIFGP
EPRFAHGLIAAVAVLIIACPCALGLATPMSIMVGVGRGASMGVLIKNAEALERFEKVDTL
VVDKTGTLTEGKPKVTALKAVAGLDEAELLRLAATLERASEHPLAAAIVAAAAERGLPLG
EAKDFDSPLGKGVTGMVDGQKLVIGSHRIMGEEGVDLSPLSAEAEALRADGATVIFVAMD
GRIGGLIAIADPVKATTPAALAALRAAGVRVVMLTGDNRTTAEAVARRLGIDEVEAEVLP
ENKAQVVTRLRQEGRIVAMAGDGVNDAPALAAADVGVAMGTGTDVAIESAGVTLLKGDLQ
GIARARQLSHATMSNIRQNLFFAFIYNAAGVPIAAGALYPLFGLLLSPIIAAAAMALSSV
SVIANSLRLRTAALGGGK