Protein Info for GFF4898 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1065 (1064 residues), 1515 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 133 to 339 (207 residues), 242.4 bits, E=1.1e-75
  • amino acids 677 to 888 (212 residues), 118.5 bits, E=9.4e-38
• PF02787: CPSase_L_D3
  • amino acids 427 to 505 (79 residues), 94.5 bits, 9.3e-31
• PF02222: ATP-grasp
  • amino acids 687 to 857 (171 residues), 35.2 bits, E=2.9e-12
• PF02142: MGS
  • amino acids 969 to 1055 (87 residues), 75.4 bits, E=1e-24

Best Hits

Swiss-Prot: 64% identical to CARB_BUCAP: Carbamoyl-phosphate synthase large chain (carB) from Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 89% identity to aaa:Acav_2592)

MetaCyc: 69% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• superpathway of arginine and polyamine biosynthesis (15/17 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1085 amino acids)

>GFF4898 Carbamoyl-phosphate synthase large chain (EC 6.3.5.5) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MPKRTDIQTVLIIGAGPIIIGQACEFDYSGVQACKALREEGYRVVLINSNPATIMTDPAT
ADVTYIEPITWQTVEKIIAKERPLAKGGFAILPTMGGQTALNCALDLWRNGVLAKYDVEL
IGATPEAIDKAEDRLKFKDAMTKIGLGSARSGIAHSMEEAWGVQKTVGFPVVIRPSFTLG
GTGGGIAYNPEEFETICKRGLEASPTNELLIEESLLGWKEYEMEVVRDKADNCIIVCSIE
NLDPMGVHTGDSITVAPAQTLTDKEYQILRNASLAVLREIGVDTGGSNVQFSINPKDGRM
VVIEMNPRVSRSSALASKATGFPIAKVAAKLAIGYTLDELRNEITGGATPASFEPSIDYV
VTKIPRFAFEKFPTADSRLTTQMKSVGEVMAMGRTFQESFQKALRGLEVGVDGMNEKTQD
RETLERELGAPGPDRIWYVGDAFAAGWTLEEVHQFTKIDPWFLVQIEEIVKIELALEKTS
LDAIDTDTLRGLKKKGFSDRRLAKLLKTNDQAVRERRIADNIRPVYKRVDTCAAEFATDT
AYLYSTYEGHGDGECEAEPTDKKKIMVLGGGPNRIGQGIEFDYCCVHAALAMREDGYETI
MVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIVDKEKPVGVIVQYGGQTPLKLALGLEAA
GVPIIGTSPDMIDAAEDRERFQQLLHTLGLRQPPNATARAEPEALEKAAALGYPLVVRPS
YVLGGRAMEIVHEQRDLERYMREAVKVSNDSPVLLDRFLNDAIECDVDAVRDATGRVFIG
GVMEHIEQAGVHSGDSACSLPPYYLSKATVDELKRQTAAMAEGLNVVGLMNVQFAIQEVE
ENGVKKDVIFVLEVNPRASRTVPFVSKATGIQLAKVAARCMAGQSLDQQGIGAEVTPPYF
SVKEAVFPFVKFPGVDTILGPEMKSTGEVMGVGKTFGEAFVKSQLGAGTKLPTSGKVFLT
VKNSDKPRAVEIARQLVEMGFSLVATRGTAAAIAAAGVVVQTVNKVTEGRPHIVDAIKNG
DIVLVINTVEERRNAIADSRAIRTSALLARVTTFTTIAGAEAAVEGMQYLDKLGVISVQE
MHAQL