Protein Info for GFF4894 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 6 to 120 (115 residues), 151.2 bits, E=1.4e-48
• PF02882: THF_DHG_CYH_C
  • amino acids 125 to 280 (156 residues), 233.8 bits, E=6.1e-74

Best Hits

Swiss-Prot: 86% identical to FOLD_ACIAC: Bifunctional protein FolD (folD) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 86% identity to aav:Aave_2459)

MetaCyc: 60% identical to methylenetetrahydrofolate dehydrogenase [multifunctional] (Bacillus methanolicus)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• folate transformations II (plants) (8/11 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• L-histidine degradation III (4/6 steps found)
• purine nucleobases degradation II (anaerobic) (15/24 steps found)
• folate transformations I (7/13 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (4/9 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (2/10 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Protein Sequence (283 amino acids)

>GFF4894 Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTAQLIDGNALSRQLRADVSARVTALKAKGITPGLAVILVGDNPASQVYVRNKVKACEDT
GMHSVLERWPATLSEAELLARVDALNRDATIHGILVQLPLPAHIDAQKVIEAISPAKDVD
GFHIASAGALMTGMPGFWPCTPYGCMKMLESIGFELKGKHAVVIGRSNIVGKPMALMLLQ
QNATVTICHSNTQDLKAQTLQADVIVAAVGKRNVLTADMVKPGAVVLDVGMNRNDEGKLC
GDVDFDGVKNVAGWITPVPGGVGPMTITMLMVNTLESAESLAG