Protein Info for GFF4893 in Xanthobacter sp. DMC5

Annotation: Caffeyl-CoA reductase-Etf complex subunit CarE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 369 PF01012: ETF" amino acids 32 to 197 (166 residues), 137.2 bits, see alignment E=5.7e-44 PF00766: ETF_alpha" amino acids 236 to 315 (80 residues), 116.2 bits, see alignment E=5.2e-38

Best Hits

Swiss-Prot: 86% identical to FIXB_AZOC5: Protein FixB (fixB) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K03522, electron transfer flavoprotein alpha subunit (inferred from 94% identity to xau:Xaut_0141)

MetaCyc: 60% identical to quinone reductase (NADH,flavodoxin) complex electron transfer flavoprotein component beta subunit (Azotobacter vinelandii)

Predicted SEED Role

"Electron transfer flavoprotein, alpha subunit" in subsystem Acetyl-CoA fermentation to Butyrate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (369 amino acids)

>GFF4893 Caffeyl-CoA reductase-Etf complex subunit CarE (Xanthobacter sp. DMC5)
MSEPTTPKAPAAGGRASTKKELPEHFKAYKHVWVFIEQERGQVHPVSWELMGAGRKLADK
LGVELAAVVVGAEGETVRGAVAESFCYGADLAYVVADPVLTDYRNEAYTAAMTDLVNTYK
PEILLLGATTLGRDLAGAVATTLLTGLTADSTELDVDADGSLAATRPTFGGSLLCTIYTL
NYRPQMATVRPRVMPMPIRVEKPIGRVIEHKLGVVEADIVTKILNFIPDRDSNKANLAYA
DVVVAGGMGLGSPENFQLVRNLAVAMGAEYGCSRPLVQKGWVSSDRQIGQTGKTIRPRLY
IAAGISGAIQHRVGVEGADLIVAINTDKNAPIFDFAHVGIVTDAIRLLPALTEAFAKRLS
AHSSDKLAS