Protein Info for GFF4886 in Sphingobium sp. HT1-2

Annotation: Cyclohexanone monooxygenase (EC 1.14.13.22)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 490 PF00743: FMO-like" amino acids 14 to 344 (331 residues), 41.7 bits, see alignment E=2.6e-14 PF07992: Pyr_redox_2" amino acids 14 to 227 (214 residues), 49.7 bits, see alignment E=1.7e-16 PF00890: FAD_binding_2" amino acids 16 to 51 (36 residues), 23.8 bits, see alignment 1.1e-08 PF13738: Pyr_redox_3" amino acids 17 to 211 (195 residues), 53.7 bits, see alignment E=8.9e-18 PF13450: NAD_binding_8" amino acids 17 to 71 (55 residues), 44.5 bits, see alignment 6.9e-15

Best Hits

Swiss-Prot: 46% identical to Y892_MYCTU: Uncharacterized monooxygenase Rv0892 (Rv0892) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: None (inferred from 45% identity to mmi:MMAR_4641)

Predicted SEED Role

"Cyclohexanone monooxygenase (EC 1.14.13.22)" (EC 1.14.13.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.22

Use Curated BLAST to search for 1.14.13.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (490 amino acids)

>GFF4886 Cyclohexanone monooxygenase (EC 1.14.13.22) (Sphingobium sp. HT1-2)
MGEARNGKAAYRPRTAIIGAGAAGMMQAIKLREAGLDDIVIYEKANEVGGTWRENRYPGV
ACDVPAHHYNYSFEHNPGWSHRLALGGEIQSYMLKVADKYDLRRDIVFGTKVTGCRFDGR
KWHLETVEGHLDTVDFLVAATGPLHVPAFPRIEGLDDFEGAKFHSAQWMDGIDLSDKRVG
VIGSGSTGTQLIAALANQGVDLTAFIRTPQWIFPLPNRRFGDMERKIVRAMPWIGRAAGR
FYGWFFEHVFAEAVIKNGWQRKFLSSMCKWHLGRIKDPELRRKLTPPDEPLCKRMVMSTA
FYPAVSRPNVEIVRDGIVRATEQGIVDQSGRLHELDVIIFATGFDARAYHRPIELTNARG
ESLTKVWEGQTGAHLSVHVPGFPNFMLVGGPHSPRGNFSAIAYSEAIVDHIVKVIKFCAE
NGYHSIEAKPEALDEFLQKVKSAVPSTIWISGCTNWYMDENGMPETWTGTPDAYREILRE
PRYEEFKLAR