Protein Info for GFF4881 in Sphingobium sp. HT1-2

Annotation: Isovaleryl-CoA dehydrogenase (EC 1.3.8.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 388 PF02771: Acyl-CoA_dh_N" amino acids 10 to 122 (113 residues), 129.6 bits, see alignment E=1.4e-41 PF02770: Acyl-CoA_dh_M" amino acids 127 to 219 (93 residues), 94.6 bits, see alignment E=6.8e-31 PF00441: Acyl-CoA_dh_1" amino acids 231 to 379 (149 residues), 154.4 bits, see alignment E=5.2e-49 PF08028: Acyl-CoA_dh_2" amino acids 247 to 363 (117 residues), 59.7 bits, see alignment E=7.9e-20

Best Hits

Swiss-Prot: 63% identical to IVD_SOLTU: Isovaleryl-CoA dehydrogenase, mitochondrial (IVD) from Solanum tuberosum

KEGG orthology group: None (inferred from 84% identity to npp:PP1Y_Mpl5168)

MetaCyc: 60% identical to isovaleryl-CoA dehydrogenase subunit (Pseudomonas aeruginosa PAO1)
RXN0-2301 [EC: 1.3.8.4]

Predicted SEED Role

"Isovaleryl-CoA dehydrogenase (EC 1.3.8.4)" (EC 1.3.8.4)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.4

Use Curated BLAST to search for 1.3.8.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (388 amino acids)

>GFF4881 Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (Sphingobium sp. HT1-2)
MQMFDFDLGESADMIRETTCRFATDKIAPLAKRIDAEDWFPRDELWTSMGELGLHGITVD
EQDGGLGLGYLEHVIACEEIARASGAIGLSYGAHTNLCINQIARWGSPEQKAKYLPKLIS
GEHLGSLAMSEAGAGSDVVSMKLKAEATSSGYVLNGTKFWITNATYADTLVVYAKTGEGS
RGITAFIIEKNMPGFSIGQKIDKMGMRGSPTAELVFDNCEVPQENALGPVNGGVGVLMSG
LDYERAVLAGIPLGIMQACLDVVLPYVRERKQFGQPIGHFQLIQAKVADMYVALNSCRAY
IYAVARSCDAGRTTRFDAAAAILLASENTVRVANEAVQALGGAGYTKDWPVERFYRDAKL
LDIGAGTNEIRRMLIGRELIGEAERLAQ