Protein Info for GFF4876 in Sphingobium sp. HT1-2

Annotation: Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 651 PF00289: Biotin_carb_N" amino acids 8 to 114 (107 residues), 140.4 bits, see alignment E=1.2e-44 PF02786: CPSase_L_D2" amino acids 121 to 328 (208 residues), 243.4 bits, see alignment E=7.1e-76 PF07478: Dala_Dala_lig_C" amino acids 145 to 297 (153 residues), 48.6 bits, see alignment E=2.9e-16 PF02222: ATP-grasp" amino acids 147 to 298 (152 residues), 32.4 bits, see alignment E=2.9e-11 PF02785: Biotin_carb_C" amino acids 342 to 448 (107 residues), 110.7 bits, see alignment E=1.6e-35 PF00364: Biotin_lipoyl" amino acids 581 to 646 (66 residues), 48 bits, see alignment E=3.6e-16

Best Hits

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (651 amino acids)

>GFF4876 Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) (Sphingobium sp. HT1-2)
MGHRMKPFSKILIANRGEIACRVIRSAKAKGYATVAVFSDADAGARHVREADEAFRLGGG
PLRESYLSAERVLAAARATGADALHPGYGFFSENADFAEQCADAGITFIGPLPDSVRVMG
DKARSKRRMIDAGVPCVPGYEGDEQDDALFVREADRIGYPVMVKASAGGGGRGMRLVHER
SDLPAALASARSEAKNAFGDDRLLLERAVVDARHVEIQVFGDTFGSVVHLGERDCSVQRR
HQKVIEEAPSPAVDQDLRQRMGEAAVRAAAAIDYVGAGTVEFLLGGDGQFYFLEMNTRLQ
VEHPVTECVTGIDLVDLQIDVAAGLPLPFSQDDVTLTGHAIEVRLYAEDPRDQFLPQTGV
ATVWEPATGEGIRVDHGLVAGQVISPFYDPMIAKIIAMGANREEALRRLVRAVRETHLLG
VATNREFLLDALSIDEFRDGGATTAFIGKHYGDGFQPREGDSAAIWLAAILATETTRAGW
SSNAVQAHPISLSSDGVVMNVRVARTGHRWSAEVDGEVASVAIVERDNVHVRFEIDGLVR
RAVYLFSDDGISIDIGGRAYRFEDMTYRAASRASAGGDGVLRAPMSGAVTSIRVSPDQAV
SRGDVLGVLEAMKMEHQIVAPISGTVATVAVTAGQQVGARDILFVIKGEPA