Protein Info for GFF4872 in Sphingobium sp. HT1-2

Annotation: Methylmalonyl-CoA mutase (EC 5.4.99.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01642: MM_CoA_mutase
  • amino acids 34 to 555 (522 residues), 502.8 bits, E=5.4e-155
• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 35 to 556 (522 residues), 444.5 bits, E=2.1e-137

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• propanoyl CoA degradation I (3/3 steps found)
• 3-hydroxypropanoate cycle (10/13 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• methylaspartate cycle (13/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (12/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (12/18 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (4/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 5.4.99.2

Use Curated BLAST to search for 5.4.99.2

Protein Sequence (558 amino acids)

>GFF4872 Methylmalonyl-CoA mutase (EC 5.4.99.2) (Sphingobium sp. HT1-2)
MTSTGTKTEDSAYERWQQTVAARYKDRSFVARNRSGVEVEPVYGGLDRMGNEAEMPGQYP
YTRGIYPIHYQYQPWMDLQIIGFGLPAQTRERMDLLLEQGGSKGYFDREAYNFIFDMPTS
MGYDPDHPGVRGSIGDCGISVCKASDYEVLLAGKDLAKTTVSLVLNAGSPAMLGLYLAAA
KRRGFAFDTLGGNLTNYIWDFFGHSGGTNFSPRGSYRLVADIAAYCSQHVPLWNPLTISE
HNICEAGATNVQAVAYSIAAIIAVNEECVKLGIEPDSVVPRFGFHVRYGENFFEEVAKTR
ALRRVYAKVNRERFGCKKSGSLQARIHAQTAGSLLTVQQPLNNLVRNAYGALAAVLSGAN
GMTINAYDEALGLPTEEAVTLSLRTSQIIAEETGAVHVSDPFGGSYYIESLTDEIERRVF
EIIADIDDRGGLIACIESGWVKQQVSAAAYQWRQEIESGARTMVGVNKYTTDEPEQYAVF
QPDPEAARLAIEDIERHRRDRDAARCSEALDKLRRAAVDVSEGRAIGSVMENLVAAAEAD
ATLGEMQAILHDVFGRNK